Effect of Abutilon indicum (L) Extract on Adipogenesis, Lipolysis and Cholesterol Esterase in 3T3-L1 Adipocyte Cell Lines.

IF 1.5 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Indian Journal of Clinical Biochemistry Pub Date : 2023-01-01 Epub Date: 2022-04-06 DOI:10.1007/s12291-022-01022-2

Lavanya Lakshminarayana, V Veeraraghavan, Kuruvalli Gouthami, Renuka Srihari, Prashantha Chowdadenahalli Nagaraja

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引用次数: 0

Abstract

Abutilon indicum (L) is an Indian traditional plant used for the treatment of diabetes and heart diseases. The present study is to evaluate the functional of A. indicum leaf extract as insulin like character to inhibit lipolysis and stimulates Adipogenesis activity. The ability of the A. indicum leaf extract in anti-obesity effect of Adipogenesis, lipolysis and cholesterol esterase functions can be predicted by using 3T3-L1 adipocyte cell lines. Substances were isolated from A. indicum leaves and the double filtered crude sample were used for Adipogenesis, lipolysis and cholesterol esterase activity using 3T3-L1 adipocytes at different concentrations. We used differential media-I, differential media-II and maintenance media (MM1) at concentrations of 20, 40, 60, 80, 100, 200 and 400 µg/mL respectively. In addition to the extract, there is a significance increase in glycerol release (p < 0.001) compared with crude and reference compounds. Cholesterol esterase activity predicts the IC₅₀ = 27.11 µg/mL of orlistat positive control compare with IC₅₀ = 8.158 µg/mL of crude extract. Based on the observation, A. indicum leaf extract can promotes lipolysis and differentiated adipocytes. It is potentially used as adjuvant in the treatment of Type 2 diabetes.

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Abutilon indicum (L) 提取物对 3T3-L1 脂肪细胞系脂肪生成、脂肪分解和胆固醇酯酶的影响

Abutilon indicum（L）是一种印度传统植物，用于治疗糖尿病和心脏病。本研究旨在评估苘麻叶提取物抑制脂肪分解和刺激脂肪生成的功能。通过使用 3T3-L1 脂肪细胞系，可以预测鸦胆子叶提取物在脂肪生成、脂肪分解和胆固醇酯化酶功能方面的抗肥胖能力。从 A. indicum 叶中分离出的物质和双层过滤的粗样品被用于不同浓度下 3T3-L1 脂肪细胞的脂肪生成、脂肪分解和胆固醇酯化酶活性。我们使用了浓度分别为 20、40、60、80、100、200 和 400 µg/mL 的差异培养基-I、差异培养基-II 和维持培养基 (MM1)。除提取物外，甘油释放量也显著增加（奥利司他阳性对照的 IC50 = 27.11 µg/mL 与粗提取物的 IC50 = 8.158 µg/mL 相比）。根据观察结果，A. indicum 叶提取物可促进脂肪分解和脂肪细胞分化。它有可能被用作治疗 2 型糖尿病的辅助药物。

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来源期刊

Indian Journal of Clinical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

4.50

自引率

4.80%

发文量

期刊介绍： The primary mission of the journal is to promote improvement in the health and well-being of community through the development and practice of clinical biochemistry and dissemination of knowledge and recent advances in this discipline among professionals, diagnostics industry, government and non-government organizations. Indian Journal of Clinical Biochemistry (IJCB) publishes peer reviewed articles that contribute to the existing knowledge in all fields of Clinical biochemistry, either experimental or theoretical, particularly deal with the applications of biochemistry, molecular biology, genetics, biotechnology, and immunology to the diagnosis, treatment, monitoring and prevention of human diseases. The articles published also include those covering the analytical and molecular diagnostic techniques, instrumentation, data processing, quality assurance and accreditation aspects of the clinical investigations in which chemistry has played a major role, or laboratory animal studies with biochemical and clinical relevance.