Synchrotron-based infrared microspectroscopy of polymeric nanoparticles and skin: Unveiling molecular interactions to enhance permeation

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemistry and Physics of Lipids Pub Date : 2022-11-01 DOI:10.1016/j.chemphyslip.2022.105254

Sofia A. Costa Lima , Ana Isabel Barbosa , Cláudia Nunes , Ibraheem Yousef , Salette Reis

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引用次数: 1

Abstract

The application of nanoparticles as permeation enhancers in skin drug delivery is a growing research field. However, the mechanisms of nanoparticles’ interaction with the skin structure are still unknown. Fucoidan/chitosan nanoparticles have demonstrated several physicochemical and biological advantages, among which is the enhancement of skin permeation. This study aims to elucidate permeation enhancement mechanisms using synchrotron-based Fourier Transform Infrared Microspectroscopy (SR-FTIRM) combined with multivariate analysis and in vitro skin permeation assay. Given the molecular weight influence on chitosan’s properties, the nanoparticles-skin interactions were evaluated with nanoparticles produced using low- and medium-molecular-weight chitosan. Chemical maps and spectral analysis revealed that fucoidan/chitosan nanoparticles induced changes in the lipids and protein regions. Inter-sample spectral differences were identified using principal component analysis. Low molecular weight fucoidan/ chitosan nanoparticles caused changes in the skin lipids’ lateral packing and structure at the stratum corneum layer towards a less ordered state and higher fluidity, and no evidence was found on proteins structure. The opposite was revealed for medium molecular weight fucoidan/chitosan nanoparticles, which induced changes in the secondary structure of keratin and altered lipid structure to an ordered and dense conformation. In vitro permeation assays with Franz diffusion cells correlate with the observed changes in the skin lipid and protein structure with enhanced skin permeation of a hydrophilic molecule incorporated within the fucoidan/chitosan nanoparticles. The findings of this study unveil molecular changes in the skin structure induced by the nanoparticles only possible with the application of the powerful and precise SR-FTIRM technique. This knowledge allows the design of nanoparticles towards an internalization pathway determining their fate within the skin structure.

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基于同步加速器的聚合物纳米颗粒和皮肤的红外微光谱学:揭示分子相互作用以增强渗透

纳米颗粒作为促渗透剂在皮肤给药中的应用是一个新兴的研究领域。然而，纳米颗粒与皮肤结构相互作用的机制尚不清楚。岩藻聚糖/壳聚糖纳米颗粒具有多种物理化学和生物学优势，其中之一是增强皮肤渗透。本研究旨在利用基于同步加速器的傅里叶变换红外微光谱(SR-FTIRM)，结合多变量分析和体外皮肤渗透试验，阐明皮肤渗透增强机制。考虑到分子量对壳聚糖性能的影响，利用中、低分子量壳聚糖制备纳米颗粒，对纳米颗粒与皮肤的相互作用进行了评价。化学图谱和光谱分析显示，岩藻聚糖/壳聚糖纳米颗粒诱导了脂质和蛋白质区域的变化。利用主成分分析鉴定样品间光谱差异。低分子量岩藻聚糖/壳聚糖纳米颗粒使皮肤角质层脂质侧向堆积和结构向低有序、高流动性的方向变化，而对蛋白质结构没有影响。中等分子量的岩藻聚糖/壳聚糖纳米颗粒则相反，它诱导角蛋白二级结构的改变和脂质结构的改变，使其变为有序和致密的构象。Franz扩散细胞的体外渗透实验与观察到的皮肤脂质和蛋白质结构的变化相关，这些变化与加入岩藻聚糖/壳聚糖纳米颗粒的亲水性分子的皮肤渗透增强有关。这项研究的发现揭示了纳米颗粒引起的皮肤结构的分子变化，只有应用强大而精确的SR-FTIRM技术才有可能。这一知识使得纳米颗粒的设计朝着内化途径决定他们的命运在皮肤结构。

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来源期刊

Chemistry and Physics of Lipids 生物-生化与分子生物学

CiteScore

7.60

自引率

2.90%

发文量

审稿时长

40 days

期刊介绍： Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.