Immunological characteristics of human umbilical cord mesenchymal stem cells after hepatogenic differentiation.

IF 1 4区医学 Q4 ENGINEERING, BIOMEDICAL

Bio-medical materials and engineering Pub Date : 2023-01-01 DOI:10.3233/BME-211331

Yanfeng Yin, Zongqiang Hu, Zheng Guan, Sha Lv, Yiyin Wang, Wenjun Su, Chuntao Yan, Yiting Wang, Li Ma, Jie Jiang, Shiming Feng, Li Li

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引用次数: 0

Abstract

Background: Acute liver failure is one of the most intractable clinical problems. The use of bioartificial livers may solve donor shortage problems. Human umbilical cord mesenchymal stem cells (hUCMSCs) are an excellent seed cell choice for artificial livers because they change their characteristics to resemble hepatocyte-like cells (HLCs) following artificial liver transplantation.

Objective: This study aimed to determine whether the immunological characteristics of hUCMSCs are changed after being transformed into hepatocyte-like cells.

Methods: HUCMSCs were isolated by the adherent method. The following hUCMSC surface markers were detected using flow cytometry: CD45, CD90, CD105, CD34, and octamer-binding transcription factor 4 (OCT-4). Functional detection of adipogenic differentiation was performed. The hUCMSCs were cultured in complete medium (control group) or induction medium (induction group), and flow cytometry was used to detect cell surface markers. Peritoneal lavage fluid was collected after intraperitoneal injection of 1 × 106 cells/mouse over 40 minutes. The leukocyte count, labeled CD45, CD3, CD4 and CD8 antibodies, and flow detection of T lymphocyte subsets were determined using the peritoneal lavage fluid.

Results: Using phenotypic and functional identification, hUCMSCs were successfully isolated using a two-step induction method. The surface markers of the hUCMSCs cells changed after HLC induction. In vivo immune results showed that hUCMSCs and HLsC induced leukocyte production.

Conclusion: Hepatic induction of hUCMSCs changes their cell surface markers. Both HLCs and hUCMSCs cause leukocytosis in vivo, but the immune response induced by HLCs is slightly stronger.

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人脐带间充质干细胞肝源性分化后的免疫学特性。

背景:急性肝衰竭是最棘手的临床问题之一。使用生物人工肝脏可以解决供体短缺的问题。人脐带间充质干细胞(hUCMSCs)是人工肝的优良种子细胞选择，因为它们在人工肝移植后改变其特征，类似于肝细胞样细胞(hlc)。目的:研究humscs转化为肝细胞样细胞后，其免疫学特性是否发生改变。方法:采用贴壁法分离HUCMSCs。流式细胞术检测以下hUCMSC表面标记物:CD45、CD90、CD105、CD34和OCT-4。进行成脂分化功能检测。将hUCMSCs分别置于完全培养液(对照组)或诱导培养液(诱导组)中培养，流式细胞术检测细胞表面标记物。腹腔注射1 × 106个细胞/只，40分钟后取腹腔灌洗液。采用腹腔灌洗液检测白细胞计数、标记CD45、CD3、CD4和CD8抗体以及T淋巴细胞亚群的流量检测。结果:通过表型和功能鉴定，采用两步诱导法成功分离出hUCMSCs。hplc诱导后，hUCMSCs细胞表面标志物发生变化。体内免疫结果显示，hUCMSCs和HLsC诱导了白细胞的产生。结论:肝诱导hUCMSCs改变其细胞表面标记物。在体内，hlc和hUCMSCs都能引起白细胞增多，但hlc诱导的免疫反应略强。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bio-medical materials and engineering 工程技术-材料科学：生物材料

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.