The Dopamine Gene Receptors (DRD_1-5) Expression Alteration in Psoriasis Patients.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-01-01 DOI:10.2174/2772270816666220629112414

Malihe Mohamadian, Hossein Mortazavi, Mina Makvand, Fatemeh Ahangari, Hasem Ahangari

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引用次数: 0

Abstract

Background: Psoriasis is a chronic inflammatory autoimmune disease that is considered linked to genetic and environmental factors such as stress. Since the neurotransmitter dopamine has a close association with stress configuration, it can be a candidate for relieving psoriasis representation. In addition to the CNS, immune cells can play a decisive role in regulating immune functions through dopamine synthesis and the expression of its receptors. Altered response of immune cells to dopamine as well as a distorted expression of dopamine receptors (DRs) in immune cells have been reported in some chronic inflammatory conditions.

Objective: This study aims the evaluation of dopamine receptor (DR1-DR5) gene expression in mononuclear blood cells of psoriatic patients in comparison with normal individuals.

Methods: We isolated peripheral mononuclear cells (PBMCs) from blood samples followed by total RNA extraction, cDNA synthesis, and real-time PCR using specific primer pairs.

Results: We found that all types of DRs are expressed in the PBMCs of normal and psoriatic individuals. We also concluded that compared to controls, DR2 and DR4 were overexpressed in psoriasis patients while DR3 was low-expressed.

Conclusion: Increased expression of DR2 and DR4 along with decreased expression of DR3 in PBMCs of psoriasis patients not only provide new insight into the pathogenesis of psoriasis but may also be effective in designing future therapeutic strategies attributable to psoriasis.

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银屑病患者多巴胺基因受体(DRD1-5)表达改变

背景:银屑病是一种慢性炎症性自身免疫性疾病，被认为与遗传和环境因素(如压力)有关。由于神经递质多巴胺与压力配置密切相关，它可以作为缓解牛皮癣表现的候选物。除中枢神经系统外，免疫细胞通过多巴胺的合成及其受体的表达在调节免疫功能中起决定性作用。免疫细胞对多巴胺的反应改变以及免疫细胞中多巴胺受体(DRs)的扭曲表达已被报道在一些慢性炎症条件下。目的:比较银屑病患者与正常人单核血细胞中多巴胺受体(DR1-DR5)基因表达的变化。方法:从血样中分离外周血单核细胞(peripheral mononuclear cells, PBMCs)，提取总RNA，合成cDNA，利用特异性引物对进行实时PCR。结果:我们发现所有类型的dr在正常和银屑病个体的PBMCs中均有表达。我们还得出结论，与对照组相比，银屑病患者中DR2和DR4过表达，而DR3低表达。结论:银屑病患者PBMCs中DR2和DR4的表达升高，DR3的表达降低，不仅为银屑病的发病机制提供了新的认识，而且可能为银屑病的治疗策略设计提供了有效的依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.