VEGF gene polymorphisms in Iranian patients with intracranial glioblastoma.

IF 1.3 4区 医学 Q4 CLINICAL NEUROLOGY
Journal of neurosurgical sciences Pub Date : 2024-10-01 Epub Date: 2023-01-18 DOI:10.23736/S0390-5616.22.05832-5
Sara Hanaei, Hanieh Mojtahedi, Mohamad Namvar, Arad Iranmehr, Reyhaneh Safaei, Azadehsadat Razavi, Marzie Esmaeili, Maryam Sadr, Arezou Rezaei, Maryam Edalatfar, Hamidreza Khayat Kashani, Mohsen Sadeghi-Naini, Farzaneh Darbeheshti, Jaber Gharehdaghi, Mehdi Forouzesh, Abdolali Ebrahimi, Nima Rezaei
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引用次数: 0

Abstract

Background: Glioblastoma is one of the most common malignant brain tumors in adults with poor prognosis. Neovascularization is one of the characteristics of these tumors, which is associated with overexpression of vascular endothelial growth factor (VEGF). Accordingly, single nucleotide polymorphisms of this gene could play an important role in structural and functional alterations leading to overexpression of this gene in GBM.

Methods: A total number of 49 patients with GBM and 50 healthy controls were included in the current study. The Genomic DNA was extracted from brain tumor/tissue samples, and after purification assessment, the alleles, and genotypes of rs3025039 and rs2010963 polymorphisms of the VEGF gene were investigated using T-ARMS-PCR.

Results: The "T" allele of rs3025039 was 2.79 times more frequent in GBM patients compared to controls (P=0.01). Moreover, the "CT" genotype was 2.83 times more common among patients (P=0.015), while the "CC" was more frequent in controls (P=0.009). The mean overall survival was significantly different between three genotypes of rs3025039, with the longest survival time in "CT" genotype (15.10±5.21, P=0.041). Besides, rs2010963, was significantly associated with GBM occurrence, with the "G" allele being 1.96 times more frequent in patients (P=0.01), as well as the "GG" genotype, which was 7.87 times more common in patients (P<0.001).

Conclusions: Polymorphisms of VEGF could potentially play a role in pathogenesis of GBM, as the allele and genotype distributions of rs3025039 and rs2010963 SNPs were significantly associated with GBM occurrence.

伊朗颅内胶质母细胞瘤患者的 VEGF 基因多态性。
背景:胶质母细胞瘤是成人中最常见的恶性脑肿瘤之一,预后较差。血管新生是这些肿瘤的特征之一,这与血管内皮生长因子(VEGF)的过度表达有关。因此,该基因的单核苷酸多态性可能在导致 GBM 中该基因过度表达的结构和功能改变中发挥重要作用:本研究共纳入 49 名 GBM 患者和 50 名健康对照者。从脑肿瘤/组织样本中提取基因组 DNA,经纯化评估后,使用 T-ARMS-PCR 检测 VEGF 基因 rs3025039 和 rs2010963 多态性的等位基因和基因型:结果:rs3025039的 "T "等位基因在GBM患者中的频率是对照组的2.79倍(P=0.01)。此外,"CT "基因型在患者中的发生率为 2.83 倍(P=0.015),而 "CC "基因型在对照组中的发生率更高(P=0.009)。rs3025039的三种基因型的平均总生存期有明显差异,其中 "CT "基因型的生存期最长(15.10±5.21,P=0.041)。此外,rs2010963 与 GBM 的发生显著相关,"G "等位基因在患者中的发生率为 1.96 倍(P=0.01),而 "GG "基因型在患者中的发生率为 7.87 倍(PConclusions:由于rs3025039和rs2010963 SNP的等位基因和基因型分布与GBM的发生显著相关,VEGF的多态性可能在GBM的发病机制中起潜在作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of neurosurgical sciences
Journal of neurosurgical sciences CLINICAL NEUROLOGY-SURGERY
CiteScore
3.00
自引率
5.30%
发文量
202
审稿时长
>12 weeks
期刊介绍: The Journal of Neurosurgical Sciences publishes scientific papers on neurosurgery and related subjects (electroencephalography, neurophysiology, neurochemistry, neuropathology, stereotaxy, neuroanatomy, neuroradiology, etc.). Manuscripts may be submitted in the form of ditorials, original articles, review articles, special articles, letters to the Editor and guidelines. The journal aims to provide its readers with papers of the highest quality and impact through a process of careful peer review and editorial work.
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