Genomic insight into Chryseobacterium turcicum sp. nov. and Chryseobacterium muglaense sp. nov. isolated from farmed rainbow trout in Turkey

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Izzet Burcin Saticioglu , Hilal Ay , Soner Altun , Muhammed Duman
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引用次数: 2

Abstract

Four strains, designated as C-2, C-17T, C-39T and Ch-15, were isolated from farmed rainbow trout samples showing clinical signs during an investigation for a fish-health screening study. The pairwise 16S rRNA gene sequence analysis showed that strain C-17T shared the highest identity level of 98.1 % with the type strain of Chryseobacterium piscium LMG 23089T while strains C-2, C-39T and Ch-15 were closely related to Chryseobacterium balustinum DSM 16775T with an identity level of 99.3 %. A polyphasic approach involving phenotypic, chemotaxonomic and genome-based analyses was employed to determine the taxonomic provenance of the strains. The overall genome relatedness indices including dDDH and ANI analyses confirmed that strains C-2, C-17T, C-39T and Ch-15 formed two novel species within the genus Chryseobacterium. Chemotaxonomic analyses showed that strains C-17T and C-39T have typical characteristics of the genus Chryseobacterium by having phosphatidylethanolamine in their polar lipid profile, MK-6 as only isoprenoid quinone and the presence of iso-C15:0 as major fatty acid. The genome size and G + C content of the strains ranged between 4.4 and 5.0 Mb and 33.5 – 33.6 %, respectively. Comprehensive genome analyses revealed that the strains have antimicrobial resistance genes, prophages and horizontally acquired genes in addition to secondary metabolite-coding gene clusters. In conclusion, based on the polyphasic analyses conducted on the present study, strains C-17T and C-39T are representatives of two novel species within the genus Chryseobacterium, for which the names Chryseobacterium turcicum sp. nov. and Chryseobacterium muglaense sp. nov. with the type strains C-17T (=JCM 34190T = KCTC 82250T) and C-39T (=JCM 34191T = KCTC 822251T), respectively, are proposed.

从土耳其养殖虹鳟鱼中分离的turcicum黄杆菌和muglense黄杆菌的基因组分析
在鱼类健康筛查研究的调查中,从显示临床症状的养殖虹鳟鱼样本中分离出四种菌株,命名为C-2、C-17T、C-39T和Ch-15。成对16S rRNA基因序列分析表明,菌株C-17T与豆状Chrysobacterium piscium LMG 23089T的同一性水平最高,为98.1%,而菌株C-2、C-39T和Ch-15与栏杆状Chryseobacterium railinum DSM 16775T的同同一性程度为99.3%,采用化学组学和基于基因组的分析来确定菌株的分类来源。包括dDDH和ANI分析在内的总体基因组相关性指数证实,菌株C-2、C-17T、C-39T和Ch-15在Chrysobacterium属中形成了两个新物种。化学分类学分析表明,菌株C-17T和C-39T具有典型的Chrysobacterium属特征,其极性脂质图谱中含有磷脂酰乙醇胺,MK-6仅为类异戊二烯醌,异C15:0为主要脂肪酸。菌株的基因组大小和G+C含量分别在4.4至5.0 Mb和33.5至33.6%之间。综合基因组分析显示,这些菌株除了次级代谢产物编码基因簇外,还具有抗微生物基因、原噬菌体和水平获得基因。总之,基于对本研究进行的多相分析,菌株C-17T和C-39T是Chrysobacterium属中的两个新物种的代表,其名称分别为Chrysoobacterium turcicum sp.nov.和Chrysocterium muglaense sp.nov,提出。
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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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