Description of a moderately acidotolerant and aerotolerant anaerobic bacterium Acidilutibacter cellobiosedens gen. nov., sp. nov. within the family Acidilutibacteraceae fam. nov., and proposal of Sporanaerobacteraceae fam. nov. and Tepidimicrobiaceae fam. nov.

IF 3.3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology Pub Date : 2023-01-01 DOI:10.1016/j.syapm.2022.126376

Hui Fan , Jiang Li , Weidong Wu , Rui Chen , Min Yang , Yinggang Zhang , Lulu Cong , Lirong Dai , Yu Deng , Lei Cheng , Shichun Ma

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引用次数: 4

Abstract

A Gram-stain positive, moderately thermophilic, acidotolerant and aerotolerant anaerobic bacterium, designated JN-28 ^T, was isolated from the pit mud of Chinese strong-flavor liquor. Growth was observed at 25–50 °C and pH 5.5–8.0 in the presence of 0–25 g l⁻¹ NaCl (optimally at 45 °C, pH 6.0, without NaCl). Strain JN-28 ^T was heterotrophic, requiring yeast extract for growth. The major cellular fatty acids were iso-C_15:0 and C_14:0. The DNA G + C content of genomic DNA was 33.54 mol%. The strain was resistant to vancomycin (10 mg l⁻¹). Genome analysis revealed the presence of genes involved in the response to mild acid stress and oxidative stress, and resistance to vancomycin. 16S rRNA gene-based phylogenetic analysis showed that strain JN-28 ^T shares ≤ 89.3 % sequence similarity with its closest relatives Sporanaerobacter acetigenes DSM 13106 ^T and other members in the order Tissierellales. Based on phenotypic and phylogenetic characteristics, Acidilutibacter cellobiosedens gen. nov., sp. nov. is proposed for the new genus and novel species with the type strain JN-28 ^T (=CCAM 418 ^T = JCM 39087 ^T). Further phylogenetic and phylogenomic analyses suggested strain JN-28 ^T represents a novel family within the order Tissierellales, for which Acidilutibacteraceae fam. nov. is proposed. In addition, the family Tissierellaceae was reclassified, Sporanaerobacteraceae fam. nov. and Tepidimicrobiaceae fam. nov. were formally proposed. Emended description of the family Tissierellaceae is also provided.

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一种中等耐酸和空气耐受性的厌氧细菌Acidilutibacter cellobiosedens gen. nov.， sp. nov.属于Acidilutibacteraceae家族。2011年11月，提出了孢子囊菌科菌群的建议。11 .和暖皮菌科;11月。

从浓香型白酒窖泥中分离到一株革兰氏阳性、中度嗜热、耐酸、耐气厌氧菌，命名为JN-28T。在0–25 g l−1 NaCl存在下，在25–50°C和pH 5.5–8.0下观察到生长（最佳温度为45°C，pH 6.0，无NaCl）。菌株JN-28T是异养的，生长需要酵母提取物。主要细胞脂肪酸为异C15:0和C14:0。基因组DNA的DNA G+C含量为33.54 mol%。该菌株对万古霉素（10 mg l−1）具有抗性。基因组分析揭示了参与对轻度酸应激和氧化应激反应以及对万古霉素耐药性的基因的存在。基于16S rRNA基因的系统发育分析表明，菌株JN-28 T与其最接近的亲缘关系——醋孢厌氧杆菌DSM 13106T和Tissierellales目其他成员具有≤89.3%的序列相似性。根据表型和系统发育特征，提出了纤维二糖酸粘杆菌（Acidlutibacter cellobisedens gen.nov.，sp.nov.）为JN-28T（=CCAM 418 T=JCM 39087T）的新属和新种。进一步的系统发育和系统发育学分析表明，菌株JN-28 T代表了一个新的Tissierellales目家族，其中酸性乳杆菌科为其家族。nov.提出。此外，将提丝菌科重新分类为孢子厌氧菌科。nov.和Tepidimicrobiaceae fam。十一月正式提出。还提供了对提西雷科的简要描述。

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来源期刊

Systematic and applied microbiology 生物-生物工程与应用微生物

CiteScore

7.50

自引率

5.90%

发文量

审稿时长

22 days

期刊介绍： Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology: