Arsenicum album Induces Cell Cycle Arrest and Apoptosis, and Inhibits Epithelial-Mesenchymal Transition in Hormone-Dependent MCF7 Breast Cancer Cells.

IF 1.2 4区医学 Q3 INTEGRATIVE & COMPLEMENTARY MEDICINE

Homeopathy Pub Date : 2023-08-01 DOI:10.1055/s-0042-1755364

Nilanjana Basu, Manoj Garg, Chanderdeep Tandon, Bhudev Chandra Das, Simran Tandon

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引用次数: 1

Abstract

Background: Arsenic trioxide (As₂O₃) has been in therapeutic use since the 18th century for various types of cancers including skin and breast; however, it gained popularity following FDA approval for its use against acute promyelocytic leukemia. This present work was designed to evaluate the anti-cancer potential of a homeopathic potency of arsenic trioxide (Arsenicum album 6C) in hormone-dependent breast cancer.

Methods: Breast cancer cells (MCF7) were treated with Arsenicum album (Ars 6C) to evaluate its anti-proliferative and apoptotic potential. We examined the effect of Ars 6C on the cell cycle, wound healing, reactive oxygen species (ROS) generation, and modulation of expression of key genes which are aberrant in cancer.

Results: Treating breast cancer cells with Ars 6C halted the cell cycle at the sub-G0 and G2/M phases, which could be attributed to DNA damage induced by the generation of ROS. Apoptotic induction was associated with upregulation of Bax expression, with concurrent downregulation of the Bcl-2 gene. Ars 6C was also seen to reverse epithelial to mesenchymal transition and reduce the migration of breast cancer cells.

Conclusion: The findings suggest that Ars has significant anti-proliferative and apoptotic potential against breast cancer cells. Further studies are required to elucidate the mechanism by which Ars exerts its effect in the in vivo setting.

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砷相册诱导细胞周期阻滞和凋亡，抑制激素依赖性MCF7乳腺癌细胞上皮-间质转化。

背景:自18世纪以来，三氧化二砷(As2O3)一直用于治疗各种类型的癌症，包括皮肤癌和乳腺癌;然而，在FDA批准其用于治疗急性早幼粒细胞白血病后，它得到了普及。本研究旨在评估三氧化二砷(Arsenicum album 6C)在激素依赖性乳腺癌中顺势疗法的抗癌潜力。方法:用Arsenicum album (Ars 6C)处理乳腺癌细胞MCF7，观察其抗增殖和凋亡潜能。我们研究了Ars 6C对细胞周期、伤口愈合、活性氧(ROS)产生以及癌症中异常关键基因表达调节的影响。结果:用Ars 6C处理乳腺癌细胞使细胞周期在亚g0期和G2/M期停止，这可能与ROS生成诱导DNA损伤有关。凋亡诱导与Bax表达上调相关，同时Bcl-2基因下调。Ars 6C还可以逆转上皮细胞向间充质细胞的转变，并减少乳腺癌细胞的迁移。结论:Ars对乳腺癌细胞具有明显的抗增殖和抗凋亡作用。需要进一步的研究来阐明Ars在体内发挥其作用的机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Homeopathy 医学-全科医学与补充医学

CiteScore

3.40

自引率

70.60%

发文量

审稿时长

20.1 weeks

期刊介绍： Homeopathy is an international peer-reviewed journal aimed at improving the fundamental understanding and clinical practice of homeopathy by publishing relevant high-quality original research articles, reviews, and case reports. It also promotes commentary and debate on matters of topical interest in homeopathy.