Betulinic acid may modulate autophagy in renal cell carcinoma cells.

IF 1.5 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

Bratislava Medical Journal-Bratislavske Lekarske Listy Pub Date : 2023-01-01 DOI:10.4149/BLL_2023_015

Merve Nur Atas, Baris Ertugrul, Elif Sinem Iplik, Bedia Cakmakoglu, Arzu Ergen

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引用次数: 1

Abstract

Objectives: Betulinic acid is pentacyclic triterpenoid known to exert antitumor effects by modulating many cellular pathways in various human malignancies. However, its modulatory role in autophagy in renal cell carcinoma remains unclear. Here, we observed how betulinic acid affects autophagy in renal cell carcinoma cells.

Methods: After treating cells with betulinic acid, we determined the gene expression and protein levels of Beclin-1 and ATG-5 by qPCR and ELISA assay to observe its effects on autophagy.

Results: The qPCR results demonstrated that Beclin-1 expression level was low in untreated metastatic renal adenocarcinoma ACHN cells and increased in response to 25 µM and 50 µM betulinic acid treatment. ATG-5 expression level was decreased in primary clear cell renal cell carcinoma CAKI-2 cells treated with 50 µM betulinic acid. In the ELISA assay results, we observed that betulinic acid caused a decrease in Beclin-1 protein level at 25 µM concentration and in ATG-5 protein level at 50 µM concentration in CAKI-2 cells.

Conclusion: In our preliminarily study, it was concluded that the role of autophagy may differ in renal cell carcinoma cells depending on their origin and that the effects of betulinic acid on autophagy in these cells may vary accordingly (Fig. 4, Ref. 40). Text in PDF www.elis.sk Keywords: betulinic acid, autophagy, kidney, cancer, cell.

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白桦酸可能调节肾细胞癌细胞的自噬。

目的:白桦酸是一种已知的五环三萜，通过调节多种人类恶性肿瘤的细胞通路来发挥抗肿瘤作用。然而，其在肾细胞癌自噬中的调节作用尚不清楚。在这里，我们观察了白桦酸如何影响肾细胞癌细胞的自噬。方法:用白桦酸处理细胞后，采用qPCR和ELISA法检测Beclin-1和ATG-5的基因表达和蛋白水平，观察其对细胞自噬的影响。结果:qPCR结果显示，Beclin-1在未经治疗的转移性肾腺癌ACHN细胞中表达水平较低，在25µM和50µM白桦酸处理下表达水平升高。50µM白桦酸可降低原代透明细胞肾细胞癌CAKI-2细胞中ATG-5的表达水平。在ELISA检测结果中，我们观察到白桦酸在25µM浓度下导致CAKI-2细胞Beclin-1蛋白水平降低，在50µM浓度下导致ATG-5蛋白水平降低。结论:在我们的初步研究中，我们得出结论，不同来源的肾细胞癌细胞中自噬的作用可能不同，白桦酸对这些细胞自噬的影响也可能相应不同(图4,Ref. 40)。关键词:白桦酸，自噬，肾脏，癌症，细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bratislava Medical Journal-Bratislavske Lekarske Listy 医学-医学：内科

CiteScore

2.60

自引率

0.00%

发文量

185

审稿时长

3-8 weeks

期刊介绍： The international biomedical journal - Bratislava Medical Journal – Bratislavske lekarske listy (Bratisl Lek Listy/Bratisl Med J) publishes peer-reviewed articles on all aspects of biomedical sciences, including experimental investigations with clear clinical relevance, original clinical studies and review articles.