Isovincathicine from Catharanthus roseus induces apoptosis in A549 cells

IF 2.5 4区医学 Q3 CHEMISTRY, MEDICINAL

Journal of Natural Medicines Pub Date : 2023-09-05 DOI:10.1007/s11418-023-01740-8

Mizuki Hiroki, Aishanjiang Abulikemu, Chihiro Totsuka, Yusuke Hirasawa, Toshio Kaneda, Hiroshi Morita

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Abstract

A dimeric indole alkaloid, isovincathicine consisting of an aspidosperma type and modified iboga with C-7–C-20 connection type skeletons was first isolated from Catharanthus roseus, and the structure including stereochemistry was elucidated on the basis of spectroscopic data as well as DP4 statistical analysis. Isovincathicine inhibited cell proliferation in A549 cells. We investigated the detailed mode of action of isovincathicine-induced inhibitory effects on cell proliferation in A549 cells. Flow cytometric analysis showed that isovincathicine-treated cells accumulated in the G₂ phase after 24 h, and the percentage of cells showing cell death increased after 48 h. Western blotting also showed increased expression of BimEL, an apoptosis-related protein, and decreased expression of Mcl-1 and Bcl-xL. Isovincathicine was suggested to induce apoptosis in A549 cells by a mechanism is similar to that of vinblastine.

Graphical abstract

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从长春花中提取的异长春花碱能诱导 A549 细胞凋亡。

首次从长春花中分离出了一种二聚吲哚生物碱--异长春花碱，它由aspidosperma型和具有C-7-C-20连接型骨架的改良Iboga型组成，并根据光谱数据和DP4统计分析阐明了包括立体化学在内的结构。异长春花碱能抑制 A549 细胞的增殖。我们研究了异长春花碱抑制 A549 细胞增殖的详细作用模式。流式细胞分析表明，异长春花碱处理的细胞在 24 小时后积累到 G2 期，48 小时后细胞死亡的百分比增加。这表明异长春新碱诱导 A549 细胞凋亡的机制与长春新碱相似。

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来源期刊

Journal of Natural Medicines 医学-药学

CiteScore

6.90

自引率

3.00%

发文量

审稿时长

1.7 months

期刊介绍： The Journal of Natural Medicines is an international journal publishing original research in naturally occurring medicines and their related foods and cosmetics. It covers: -chemistry of natural products -biochemistry of medicinal plants -pharmacology of natural products and herbs, including Kampo formulas and traditional herbs -botanical anatomy -cultivation of medicinal plants. The journal accepts Original Papers, Notes, Rapid Communications and Natural Resource Letters. Reviews and Mini-Reviews are generally invited.