Effect of brief exposures of anesthesia on thermotolerance and metabolic rate of the spotted-wing fly, Drosophila suzukii: Differences between sexes?

IF 2.3 2区 农林科学 Q1 ENTOMOLOGY
Florencia A. Putero , Julian Mensch , Pablo E. Schilman
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Abstract

The spotted-wing fly, Drosophila suzukii, is a world-wide pest insect for which there is increasing interest in its physiological traits including metabolism and thermotolerance. Most studies focus only on survival to different time exposures to extreme temperatures, mainly in female flies. In addition, it has not been tested yet how anesthesia affects these measurements. We analyzed the effects of anesthesia by brief exposures to cold, anoxia by CO2 or N2 on three standard thermotolerance assays, as well as the aerobic metabolic rate in both sexes. For heat tolerance we measured CTmax by thermolimit respirometry, and CTmin and chill-coma recovery time for cold tolerance. Aerobic metabolism was calculated by CO2 production of individual flies in real time by open flow respirometry. Results showed that females have a significantly higher V̇CO2 for inactive (at 25 °C) and maximum metabolic rate than males. This difference is mainly explained by body mass and disappears after mass correction. Males had a more sensitive MR to temperature than females showed by a significantly higher Q10 (2.19 vs. 1.98, for males and females, respectively). We observed a significantly lower CTmin (X2 = 4.27, P = 0.03) in females (3.68 ± 0.38 °C) than males (4.56 ± 0.39 °C), although we did not find significant effects of anesthesia. In contrast, anesthesia significantly modifies CTmax for both sexes (F3,62 = 7.86, P < 0.001) with a decrease of the CTmax in cold-anesthetized flies. Finally, we found a significantly higher CTmax in females (37.87 ± 0.07 °C) than males (37.36 ± 0.09 °C). We conclude that cold anesthesia seems to have detrimental effects on heat tolerance, and females have broader thermotolerance range than males, which could help them to establish in invaded temperate regions with more variable environmental temperatures.

Abstract Image

短暂暴露麻醉对斑翅果蝇耐热性和代谢率的影响:性别差异?
斑翅蝇是一种世界性害虫,其代谢和耐热性等生理特性越来越受到人们的关注。大多数研究只关注不同时间暴露在极端温度下的存活率,主要是雌性苍蝇。此外,尚未测试麻醉对这些测量的影响。我们分析了短暂暴露于寒冷、CO2或N2缺氧的麻醉对三种标准耐热性测定的影响,以及两性的有氧代谢率。对于耐热性,我们通过热极限呼吸法测量了CTmax,并测量了冷耐受性的CTmin和冷昏迷恢复时间。有氧代谢是通过开流呼吸法实时计算单个苍蝇的二氧化碳产生量。结果表明,雌性的无活性(在25°C下)V̇CO2和最大代谢率明显高于雄性。这种差异主要由身体质量来解释,并在质量校正后消失。雄性对温度的MR比雌性更敏感,Q10明显更高(雄性和雌性分别为2.19和1.98)。我们观察到女性(3.68±0.38°C)的CTmin(X2=4.27,P=0.03)显著低于男性(4.56±0.39°C),尽管我们没有发现麻醉的显著影响。相反,麻醉显著改变了两性的CTmax(F3,62=7.86,P<;0.001),同时降低了冷麻醉苍蝇的CTmax。最后,我们发现女性的CTmax(37.87±0.07°C)明显高于男性(37.36±0.09°C。
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来源期刊
Journal of insect physiology
Journal of insect physiology 生物-昆虫学
CiteScore
4.50
自引率
4.50%
发文量
77
审稿时长
57 days
期刊介绍: All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.
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