Relation of CRP gene variants to altered risk of Helicobacter pylori - associated chronic gastritis: A case-control study in Tunisia

IF 2.3 3区 生物学 Q3 BIOCHEMICAL RESEARCH METHODS
Mouna Stayoussef , Sabrina Zidi , Perizat Kanabekova , Leila Mouellhi , Wassim Y. Almawi , Besma Yaacoubi-Loueslati
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引用次数: 0

Abstract

Background

We investigated the association between CRP variants and chronic gastritis in H. pylori-infected patients at the allele, genotype, and haplotype levels. This was also assessed according to serum hs-CRP levels.

Methods

Study subjects consisted of 77 H. pylori-infected patients and 96 H. pylori-negative controls. Genotyping of the CRP rs1572970, rs876537, rs2794520, rs2808630, rs1130864, rs1417938, rs7553007, and rs4285692 variants were analyzed by real-time PCR.

Results

Significantly higher MAF and increased risk of chronic gastritis were associated with rs1130864, rs1417938, and rs7553007, which persisted after controlling for key covariates. Significant differences in the genotype distribution of rs1130864, rs1417938, and rs7553007 were also seen between H. pylori-infected patients and healthy controls. Increased risk of H. pylori-associated chronic gastritis was associated with carriage of rs1130864 C/T, and more with T/T genotype carriers, as well as with rs1417938 T/A and A/A genotype carriers. Functionally, the distribution of rs1130864 and rs1417938 genotypes were significantly different between H. pylori-infected patients and controls in the low hs-CRP (<6 mg/L) group. CRP haplotype analysis identified Block 1 (rs1572970, rs876537, rs2794520), and Block 2 (rs2808630, rs1130864, rs1417938) associated with H. pylori infection. Haplotypes ACC (Block 1) and TTA and TTT (Block 2) were positively associated with H. pylori-associated chronic gastritis with low hs-CRP levels.

Conclusion

Altered serum levels of hs-CRP, stemming in part from the presence of specific genetic variants in CRP gene, modulate the risk of H. pylori infection.

CRP基因变异与幽门螺杆菌相关慢性胃炎风险改变的关系:突尼斯的一项病例对照研究
背景:我们从等位基因、基因型和单倍型水平研究了c反应蛋白变异与幽门螺杆菌感染患者慢性胃炎的关系。这也根据血清hs-CRP水平进行评估。方法研究对象为77例幽门螺杆菌感染患者和96例幽门螺杆菌阴性对照。采用实时荧光定量PCR对CRP rs1572970、rs876537、rs2794520、rs2808630、rs1130864、rs1417938、rs7553007和rs4285692变异进行基因分型分析。结果较高的MAF和增加的慢性胃炎风险与rs1130864、rs1417938和rs7553007相关,并在控制关键协变量后持续存在。rs1130864、rs1417938和rs7553007基因型分布在幽门螺杆菌感染患者和健康对照组之间也存在显著差异。幽门螺杆菌相关慢性胃炎的风险增加与携带rs1130864 C/T相关,与T/T基因型携带者、rs1417938 T/A和A/A基因型携带者相关。功能上,低hs-CRP (<6 mg/L)组幽门螺杆菌感染患者与对照组rs1130864和rs1417938基因型分布有显著差异。CRP单倍型分析发现Block 1 (rs1572970、rs876537、rs2794520)和Block 2 (rs2808630、rs1130864、rs1417938)与幽门螺杆菌感染相关。单倍型ACC (Block 1)、TTA和TTT (Block 2)与幽门螺杆菌相关性慢性胃炎伴hs-CRP水平低呈正相关。结论血清hs-CRP水平的改变可调节幽门螺杆菌感染的风险,其部分原因是CRP基因存在特定的遗传变异。
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来源期刊
Molecular and Cellular Probes
Molecular and Cellular Probes 生物-生化研究方法
CiteScore
6.80
自引率
0.00%
发文量
52
审稿时长
16 days
期刊介绍: MCP - Advancing biology through–omics and bioinformatic technologies wants to capture outcomes from the current revolution in molecular technologies and sciences. The journal has broadened its scope and embraces any high quality research papers, reviews and opinions in areas including, but not limited to, molecular biology, cell biology, biochemistry, immunology, physiology, epidemiology, ecology, virology, microbiology, parasitology, genetics, evolutionary biology, genomics (including metagenomics), bioinformatics, proteomics, metabolomics, glycomics, and lipidomics. Submissions with a technology-driven focus on understanding normal biological or disease processes as well as conceptual advances and paradigm shifts are particularly encouraged. The Editors welcome fundamental or applied research areas; pre-submission enquiries about advanced draft manuscripts are welcomed. Top quality research and manuscripts will be fast-tracked.
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