The effect of α-linolenic acid enrichment in perinatal diets in preventing high fat diet-induced SCD1 increased activity and lipid disarray in adult offspring of low density lipoprotein receptor knockout (LDLRKO) mice

IF 3
A. Leikin-Frenkel , H. Cohen , R. Keshet , R. Shnerb-GanOr , M. Kandel-Kfir , A. Harari , K.S. Hollander , A. Shaish , D. Harats , Y. Kamari
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引用次数: 1

Abstract

The present study examined the effects of maternal perinatal dietary ALA enrichment on the high fat diet (HFD)-induced lipid disarray in the adult offspring of low density lipoprotein receptor knock-out (LDLRKO) mice.

Female LDLRKO mice received, during pregnancy and lactation, isocaloric diets with either corn oil, RD, or flax oil, ALA. The weaning offspring was given a regular chow diet for a washout period of eight weeks, which was followed by HFD for eight weeks. Plasma and liver lipids and SCD1 activity were then analyzed.

The HFD-fed RD adult offspring had substantially higher plasma cholesterol levels than the HFD-fed ALA offspring (15.7 versus 9.7 mmole/l, p<0.00001) and non-alcoholic fatty liver disease (NAFLD) (65.0 versus 23.9 mg/g lipids, p<0.00001). Liver lipids oleic acid (OA) content and monounsaturated to saturated fatty acids (MUFA/SAT) ratio, were two times lower in RD compared to ALA (p<0.0001). The threefold HFD-induced SCD1 raised activity (p<0.00001), and OA produced from SA, observed in RD adult offspring were prevented by perinatal ALA.

In conclusion, the resilience of SCD1 to HFD- induced increased activity may account for the beneficial effects of perinatal ALA dietary enrichment in preventing NAFLD and hypercholesterolemia from occurring in adult LDLRKO offspring mice.

围产期日粮中α-亚麻酸富集对低密度脂蛋白受体敲除(LDLRKO)小鼠成年后代高脂饮食诱导的SCD1活性升高和脂质紊乱的影响
本研究探讨了母体围产期饮食ALA富集对低密度脂蛋白受体敲除(LDLRKO)小鼠成年后代高脂肪饮食(HFD)诱导的脂质紊乱的影响。雌性LDLRKO小鼠在怀孕和哺乳期间接受含有玉米油(RD)或亚麻油(ALA)的等热量饮食。断奶后的幼崽在8周的洗脱期给予正常的食物饮食,随后给予8周的HFD。然后分析血浆和肝脏脂质以及SCD1活性。饲喂hfd的RD成年后代血浆胆固醇水平明显高于饲喂hfd的ALA后代(15.7对9.7 mmol /l, p<0.00001)和非酒精性脂肪性肝病(NAFLD)(65.0对23.9 mg/g脂质,p<0.00001)。与ALA相比,RD的肝脂质油酸(OA)含量和单不饱和脂肪酸/饱和脂肪酸(MUFA/SAT)比低2倍(p<0.0001)。hfd诱导的SCD1活性增加了三倍(p<0.00001),并且在RD成年后代中观察到SA产生的OA被围产期ALA阻止。综上所述,SCD1对HFD诱导的活性增加的恢复能力可能解释了围产期饮食中富集ALA对预防成年LDLRKO后代小鼠NAFLD和高胆固醇血症的有益作用。
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来源期刊
Prostaglandins, leukotrienes, and essential fatty acids
Prostaglandins, leukotrienes, and essential fatty acids Clinical Biochemistry, Endocrinology, Diabetes and Metabolism
CiteScore
5.30
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审稿时长
64 days
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