Do Colletotrichum gloeosporioides and Rhizopus stolonifer induce alkaloidal and antifungal responses in Annona muricata seedlings?

Abstract

The benzylisoquinoline alkaloids of Annona muricata have been isolated, but their physiological or ecological role is unknown. The objective was to explore whether these secondary metabolites are involved in defense against phytopathogenic fungi. To do this, the alkaloidal response of 6-leaf seedlings of A. muricata was analyzed, previously inoculated with Colletotrichum gloeosporioides and Rhizopus stolonifer. Before and after inoculation, alkaloidal extracts of roots, stems, and leaves were obtained, and the antifungal activity was evaluated in vitro. The alkaloids anonaine, reticuline, nornuciferine, assimilobine, and coreximine were identified. C. gloeosporioides caused variable increases in the production of anonaine, reticuline and nornuciferine (10-1200%), while R. stolonifer only stimulated the increase of nornuciferin and anonaine (10%) in the stems and leaves. The alkaloidal extracts of inoculated seedlings increased the antifungal activity, both against the pathogen elicitor and against the second target pathogen. These findings suggest that the alkaloids participate in the antifungal defense mechanism.