Metformin-dependent variation of microglia phenotype dictates pericytes maturation under oxygen-glucose deprivation.

IF 3.6 Q2 MEDICINE, RESEARCH & EXPERIMENTAL
Mohammad Hossein Geranmayeh, Reza Rahbarghazi, Nazli Saeedi, Mehdi Farhoudi
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引用次数: 3

Abstract

Blood-brain barrier resident cells are in the frontline of vascular diseases. To maintain brain tissue homeostasis, a series of cells are integrated regularly to form the neurovascular unit. It is thought that microglia can switch between M1/M2 phenotypes after the initiation of different pathologies. The existence of transition between maturity and stemness features in pericytes could maintain blood-brain barrier functionality against different pathologies. In the current study, the effect of metformin on the balance of the M1/M2 microglial phenotype under oxygen-glucose deprivation conditions and the impact of microglial phenotype changes on pericyte maturation have been explored. Both microglia and pericytes were isolated from the rat brain. Data showed that microglia treatment with metformin under glucose- and oxygen-free conditions suppressed microglia shifting into the M2 phenotype (CD206+ cells) compared to the control (p < .01) and metformin-treated groups (p < .05). Incubation of pericytes with microglia-conditioned media pretreated with metformin under glucose- and oxygen-free conditions or normal conditions increased pericyte maturity. These changes coincided with the reduction of the Sox2/NG2 ratio compared to the control pericytes (p < .05). Data revealed the close microglial-pericytic interplay under the ischemic and hypoxic conditions and the importance of microglial phenotype acquisition on pericyte maturation.

二甲双胍依赖性小胶质细胞表型的变化决定了氧-葡萄糖剥夺下周细胞的成熟。
血脑屏障驻留细胞处于血管疾病的第一线。为了维持脑组织的内稳态,一系列细胞有规律地整合形成神经血管单元。据认为,小胶质细胞可以在不同病理开始后在M1/M2表型之间切换。周细胞成熟和干性过渡的存在可以维持血脑屏障的功能,以对抗不同的病理。本研究探讨了在缺氧-葡萄糖剥夺条件下,二甲双胍对M1/M2小胶质细胞表型平衡的影响,以及小胶质细胞表型变化对周细胞成熟的影响。从大鼠脑中分离出小胶质细胞和周细胞。数据显示,与对照组相比,二甲双胍在无葡萄糖和无氧条件下治疗小胶质细胞可抑制小胶质细胞向M2表型(CD206+细胞)转移
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来源期刊
Tissue Barriers
Tissue Barriers MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
6.60
自引率
6.50%
发文量
25
期刊介绍: Tissue Barriers is the first international interdisciplinary journal that focuses on the architecture, biological roles and regulation of tissue barriers and intercellular junctions. We publish high quality peer-reviewed articles that cover a wide range of topics including structure and functions of the diverse and complex tissue barriers that occur across tissue and cell types, including the molecular composition and dynamics of polarized cell junctions and cell-cell interactions during normal homeostasis, injury and disease state. Tissue barrier formation in regenerative medicine and restoration of tissue and organ function is also of interest. Tissue Barriers publishes several categories of articles including: Original Research Papers, Short Communications, Technical Papers, Reviews, Perspectives and Commentaries, Hypothesis and Meeting Reports. Reviews and Perspectives/Commentaries will typically be invited. We also anticipate to publish special issues that are devoted to rapidly developing or controversial areas of research. Suggestions for topics are welcome. Tissue Barriers objectives: Promote interdisciplinary awareness and collaboration between researchers working with epithelial, epidermal and endothelial barriers and to build a broad and cohesive worldwide community of scientists interesting in this exciting field. Comprehend the enormous complexity of tissue barriers and map cross-talks and interactions between their different cellular and non-cellular components. Highlight the roles of tissue barrier dysfunctions in human diseases. Promote understanding and strategies for restoration of tissue barrier formation and function in regenerative medicine. Accelerate a search for pharmacological enhancers of tissue barriers as potential therapeutic agents. Understand and optimize drug delivery across epithelial and endothelial barriers.
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