Role of MyD88-Adaptor-Like (MAL) Gene Polymorphism rs8177374 and Cytokine (IFN-γ, TNF-α, IL-10, TGF-β) Levels in Diverse Malaria Manifestations upon P. falciparum and P. vivax Infections.

IF 1.3 4区 医学 Q4 INFECTIOUS DISEASES
Japanese journal of infectious diseases Pub Date : 2023-11-22 Epub Date: 2023-08-31 DOI:10.7883/yoken.JJID.2023.079
Asima Rani, Syed Kashif Nawaz, Muhammad Arshad, Najma Arshad
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引用次数: 0

Abstract

This study investigated the role of genetic variant rs8177374 in MAL/TIRAP gene in mediating the cytokine levels of IFN-γ, TNF-α, IL-10, and TGF-β in malaria patients due to Plasmodium falciparum or P. vivax infection. The study included human blood samples collected from patients with malaria (n = 228) and healthy controls (n = 226). P. falciparum and P. vivax groups were established based on the causative species of Plasmodium. Malaria samples were divided into mild and severe malaria groups based on the symptoms that appeared in the patients, according to the WHO criteria. In a previous study, we genotyped rs8177374 via allele specific PCR strategy. In this study, cytokine levels were estimated in the blood plasma of rs8177374 genotype samples via Sandwich Enzyme Linked Immunosorbent Assay kits. Increased IFN-γ and TNF-α levels in presence of CC genotype indicates the role of CC genotype in both severe and mild malaria groups. Enhanced IL-10 levels in the CT genotype and mild malaria groups suggest a role of CT genotype and IL-10 in the mild clinical outcomes of malaria. The rs8177374 polymorphism in MAL/TIRAP plays an important role in malaria pathogenesis.

MyD88-Adaptor-Like (MAL)基因多态性rs8177374和细胞因子(IFN-γ、TNF-α、IL-10、TGF-β)水平在恶性疟原虫和间日疟原虫感染不同疟疾表现中的作用
本研究探讨MAL/TIRAP基因变异rs8177374在恶性疟原虫或间日疟原虫感染疟疾患者体内IFN-γ、TNF-α、IL-10、TGF-β等细胞因子水平调控中的作用。该研究包括从疟疾患者(n = 228)和健康对照者(n = 226)收集的人类血液样本。根据疟原虫的致病种划分恶性疟原虫和间日疟原虫类群。根据世卫组织的标准,根据患者出现的症状将疟疾样本分为轻度和重度疟疾组。在之前的研究中,我们通过等位基因特异性PCR策略对rs8177374进行了基因分型。本研究通过Sandwich酶联免疫吸附测定试剂盒(Sandwich Enzyme Linked Immunosorbent Assay kit)测定rs8177374基因型样本血浆中细胞因子水平。CC基因型存在时IFN-γ和TNF-α水平升高表明CC基因型在重度和轻度疟疾组中的作用。CT基因型和轻度疟疾组中IL-10水平的升高表明CT基因型和IL-10在轻度疟疾临床结果中起作用。MAL/TIRAP中rs8177374多态性在疟疾发病机制中起重要作用。
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来源期刊
CiteScore
4.50
自引率
4.50%
发文量
172
审稿时长
2 months
期刊介绍: Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.
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