EFFICIENCY OF PHOTON CAPTURE BEAM TECHNOLOGY AND PHOTODYNAMIC IMPACT ON MALIGNANT AND NORMAL HUMAN CELLS IN VITRO.

V V Talko, G Y Lavrenchuk, O D Pochapinskyi, N P Atamanuk, A V Chernyshov
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引用次数: 0

Abstract

Objective: to investigate the structural and morphofunctional changes in test systems of malignant (cell lineA549) and normal (stem fibroblasts) human cells exposed to X-rays in the presence of gadoliniumcontaining photon capture agent «Dotavist» and optical light (red spectrum) in combination with «Fotolon» photosensitizer.

Methods: The continuous cell culture of normal human fibroblasts and malignant human cells technology, X-ray and red light exposure, cytological and statistical methods.

Results: Effects of the two binary radiation technologies, namely the photon capture impact on malignant cells(human nonsmall cell lung cancer cells i.e. line A-549) and normal cells (human stem fibroblasts) when incubated with gadoliniumcontaining photon capture agent «Dotavist» and photodynamic effect in the presence of «Fotolon» photosensitizer applied separately and in combination were studied in a comparative mode. Proceeding from morphofunctional characteristics (growth kinetics, proliferative and mitotic activity) of the abovementioned test systems, peculiarities of the effect on malignant and normal cells were established. Irradiation with X-rays to the 1.0, 5.0, and 10.0 Gy doses resulted in inactivation of respectively 10 %, 46 %, and 80% of the A-549 line malignant cells.Cellular irradiation to a 1.0 Gy dose in the presence of the photon capture agent «Dotavist» (10 μl/ml concentration) inhibited cell proliferation by 50 %, suppressing their mitotic activity. At a dose of 10.0 Gy in the presence of «Dotavist» the inhibition by 93 % of the growth and division of malignant cells occurred, indicating the high efficiency of binary radiation technology. The effect of two binary radiation technologies on malignant human cells (A-549 line), namely the combination of red light with «Fotolon» (0.05 mg/ml concentration) and X-ray exposure in the above doses with «Dotavist» (10 μl/ ml concentration) resulted in the death of respectively 64 %, 86 %, and 99% malignant cells. The culture of normal fibroblasts was found being more sensitive to the influence of a complex of binary radiation impact, as exposure to a dose of 10.0 Gy in the presence of «Dotavist» and «Fotolon» inactivated 100 % of cells.

Conclusion: The obtained results provide basis of preclinical evaluation of effectiveness of the combined impact of two binary technologies and drugs used in the photon capture technology and photodynamic therapy i.e. the photon capture agent «Dotavist» and «Fotolon» photosensitizer respectively.

光子捕获束技术的效率和光动力学对体外正常和恶性人体细胞的影响。
目的:研究恶性(细胞系a549)和正常(干细胞成纤维细胞)人细胞在含钆光子捕获剂“Dotavist”和光(红色光谱)与“Fotolon”光敏剂联合作用下暴露于x射线下的测试系统的结构和形态功能变化。方法:采用正常人成纤维细胞和恶性人细胞连续培养技术,x射线和红光照射,细胞学和统计学方法。结果:以比较模式研究了两种二元辐射技术对恶性细胞(人类非小细胞肺癌细胞,即a -549系)和正常细胞(人类干细胞成纤维细胞)在含钆光子捕获剂“Dotavist”和“Fotolon”光敏剂单独或联合应用时的光动力效应的影响。从上述测试系统的形态功能特征(生长动力学、增殖和有丝分裂活性)出发,确定了对恶性细胞和正常细胞的作用特点。1.0、5.0和10.0 Gy剂量的x射线照射分别导致10%、46%和80%的A-549系恶性细胞失活。在光子捕获剂“Dotavist”(10 μl/ml浓度)存在下,1.0 Gy剂量的细胞照射可抑制50%的细胞增殖,抑制其有丝分裂活性。在“Dotavist”存在的10.0 Gy剂量下,恶性细胞的生长和分裂受到93%的抑制,表明二元辐射技术的高效率。两种二元辐射技术对人类恶性细胞(A-549系)的影响,即红光与“Fotolon”(0.05 mg/ml浓度)联合照射和上述剂量的x射线与“Dotavist”(10 μl/ ml浓度)照射分别导致64%、86%和99%的恶性细胞死亡。研究发现,正常成纤维细胞的培养对复合二元辐射的影响更为敏感,因为在" Dotavist "和" Fotolon "存在的情况下,暴露于10.0 Gy的剂量,使细胞100%失活。结论:所得结果为光子捕获技术和光动力治疗中使用的两种二元技术和药物(分别为光子捕获剂Dotavist和Fotolon光敏剂)联合影响的临床前评价提供了依据。
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来源期刊
Problemy radiatsiinoi medytsyny ta radiobiolohii
Problemy radiatsiinoi medytsyny ta radiobiolohii Medicine-Radiology, Nuclear Medicine and Imaging
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