Dynamic Population of Gut Microbiota as an Indicator of Inflammatory Bowel Disease

Q2 Biochemistry, Genetics and Molecular Biology
Afsaneh Salimi, Amin Sepehr, Hossein Ajdarkosh, Shadi Aghamohamad, Maliheh Talebi, Mahdi Rohani, Mohammad Reza Pourshafie
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引用次数: 2

Abstract

Background: Inflammatory bowel disease is a chronic inflammatory disease of the gastrointestinal tract. The gut microbiota is an important factor in the pathogenesis of inflammatory bowel disease (IBD). Due to a link between the gut microbiota and IBD, studying microbiota changes using an accurate, sensitive and rapid method for detection of the disease seems necessary. This study aimed to compare the composition of gut microbiota in three groups of people, including IBD patients, cured Inflammatory bowel disease (CIBD), and healthy groups.

Methods: For this study, 45 stool samples (15 from each group) were collected. Using real-time PCR, the abundance of 11 bacterial 16S rRNA gene sequences was examined.

Results: In the IBD group, the number of three bacterial phyla, including Firmicutes, Actinobacteria, and Bacteroidetes, decreased (p < 0.01, p < 0.01, and p < 0.001, respectively), while the population of γ-Proteobacteria increased significantly (p < 0.0001). In the CIBD group, the number of Actinobacteria enhanced (p < 0.01), but that of Bacteroidetes and Firmicutes decreased (p < 0.01, and p < 0.05, respectively).

Conclusion: Findings of this study indicate that decrease in Firmicutes and increase in γ-Proteobacteria could be used as an indicator of IBD instead of employing invasive and costly detection methods such as colonoscopy and other tests.

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肠道微生物群作为炎症性肠病指标的动态种群
背景:炎症性肠病是一种慢性胃肠道炎症性疾病。肠道菌群是炎症性肠病(IBD)发病的重要因素。由于肠道微生物群与IBD之间的联系,使用一种准确、敏感和快速的疾病检测方法研究微生物群的变化似乎是必要的。本研究旨在比较三组人群的肠道微生物群组成,包括IBD患者,治愈的炎症性肠病(CIBD)和健康组。方法:本研究共收集粪便标本45份,每组15份。采用实时荧光定量PCR检测了11个细菌16S rRNA基因序列的丰度。结果:IBD组中厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes) 3门细菌数量分别减少(p < 0.01、p < 0.01、p < 0.001), γ-变形菌门(γ-Proteobacteria)数量显著增加(p < 0.0001)。CIBD组放线菌门数量增加(p < 0.01),拟杆菌门数量减少(p < 0.01),厚壁菌门数量减少(p < 0.05)。结论:本研究结果表明,厚壁菌门的减少和γ-变形菌门的增加可以作为IBD的指标,而不是采用侵入性和昂贵的结肠镜检查等检测方法。
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来源期刊
Iranian Biomedical Journal
Iranian Biomedical Journal Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
3.20
自引率
0.00%
发文量
42
审稿时长
8 weeks
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