[Structural and functional changes in rat liver mitochondria under calcium ion loading in the absence and presence of flavonoids].

Q3 Biochemistry, Genetics and Molecular Biology
I B Zavodnik, T A Kovalenia, A G Veiko, E A Lapshina, T V Ilyich, R I Kravchuk, L B Zavodnik, I I Klimovich
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引用次数: 0

Abstract

The aim of the present work was to elucidate the mechanisms of calcium ion-induced impairments of the ultrastructure and functional activity of isolated rat liver mitochondria in the absence and presence of a number of flavonoids in vitro. In the presence of exogenous Ca²⁺ (20-60 μM), mitochondrial heterogeneity in size and electron density markedly increased: most organelles demonstrated a swollen electron-light matrix, bigger size, elongated cristae and a reduced their number, a damaged native structure of the inner membrane up to its detachment, and some mitochondria showed a more electron-dense matrix (condensed mitochondria). The calcium-induced opening of the mitochondrial permeability transition pores (MPTP) resulted in the ultrastructural disturbances and in the effective inhibition of the respiratory activity of rat liver mitochondria. The flavonoids (10-25 μM) naringenin and catechin, dose-dependently inhibited the respiratory activity of mitochondria and stimulated the MPTP opening in the presence of Ca²⁺ ions. Since Ruthenium red, an inhibitor of the mitochondrial Ca²⁺ uniporter, effectively prevented Ca²⁺-induced MPTP opening both in the absence and presence of flavonoids, we hypothesized that the effect of flavonoids on the MPTP opening could be mediated by stimulation of the Ca²⁺ uniporter.

[黄酮类化合物缺失和存在时大鼠肝脏线粒体在钙离子负荷下的结构和功能变化]。
本研究旨在探讨钙离子诱导离体大鼠肝线粒体超微结构和功能活性损伤的机制。在外源Ca 2 + (20-60 μM)存在下,线粒体大小和电子密度的异质性明显增加:大多数细胞器表现为电子光基质肿胀,尺寸增大,嵴延长,数量减少,内膜的天然结构受损直至脱离,部分线粒体表现为电子密度更大的基质(浓缩线粒体)。钙诱导大鼠肝脏线粒体通透性过渡孔(MPTP)开放,引起超微结构紊乱,有效抑制大鼠肝脏线粒体的呼吸活性。黄酮类化合物(10-25 μM)柚皮素和儿茶素在ca2 +存在下呈剂量依赖性地抑制线粒体的呼吸活性,并刺激MPTP开放。由于钌红(一种线粒体Ca 2 +单转运体的抑制剂)在没有和存在黄酮类化合物的情况下都能有效阻止Ca 2 +诱导的MPTP开放,我们假设黄酮类化合物对MPTP开放的影响可以通过刺激Ca 2 +单转运体来介导。
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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
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