Hepatorenal Protective Effects of Hydroalcoholic Extract of Solidago canadensis L. against Paracetamol-Induced Toxicity in Mice.

IF 3.4 Q2 TOXICOLOGY
Omid Rahimi, Nilufar Asadi Louie, Alireza Salehi, Firouz Faed Maleki
{"title":"Hepatorenal Protective Effects of Hydroalcoholic Extract of <i>Solidago canadensis</i> L. against Paracetamol-Induced Toxicity in Mice.","authors":"Omid Rahimi,&nbsp;Nilufar Asadi Louie,&nbsp;Alireza Salehi,&nbsp;Firouz Faed Maleki","doi":"10.1155/2022/9091605","DOIUrl":null,"url":null,"abstract":"<p><p>Paracetamol (AKA acetaminophen) is a widely used drug and is used for mild to moderate pains, such as mild osteoarthritis, toothache, headache, and pain caused by minimally invasive surgeries. Despite being a harmless drug in lower doses, acetaminophen can be toxic to the liver and kidneys if overdosed and even results in death. In this study, the therapeutic effects of <i>Solidago canadensis</i> L. extract (SCE) were investigated. 48 adult male Swiss albino mice (20-30 grams) were randomly divided into six groups of 8. The control group was gavaged with normal saline every 12 hours for 6 days. The second group received paracetamol at a 500 mg/kg intraperitoneally (i.p) dose on the sixth day. The third, fourth, and fifth groups were gavaged doses of 125, 250, and 500 mg/kg of SCE every 12 hours for six days, respectively, and on the sixth day, we received paracetamol at a dose of 500 mg/kg i.p. The sixth group only received SCE every 12 hours at a dose of 1000 mg/kg via gavaging for six days. On the seventh day (24 hours after paracetamol injection), blood samples were collected to measure the serum level of creatinine, uric acid, blood urea nitrogen (BUN), total protein, albumin, alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), and total and direct bilirubin, and liver and kidney tissues were also sampled for histopathological examination. It was observed that paracetamol caused a considerable increase in the ALT, AST, ALP, uric Acid, and BUN levels (<i>P</i> < 0.01), while those in SCE-treated groups were significantly lower. In addition, various lesions in the paracetamol group were observed, while in the SCE-receiving groups, receiving prophylactic SCE inhibited the high-intense lesions such as the infiltration of inflammatory cells, hyperemia, and vacuolar degeneration, which decreased significantly in the control group in comparison with that of the paracetamol group (<i>P</i> < 0.05). In conclusion, SCE can have substantial protective effects against paracetamol's hepatorenal toxicity.</p>","PeriodicalId":17421,"journal":{"name":"Journal of Toxicology","volume":"2022 ","pages":"9091605"},"PeriodicalIF":3.4000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9789909/pdf/","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Toxicology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1155/2022/9091605","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"TOXICOLOGY","Score":null,"Total":0}
引用次数: 5

Abstract

Paracetamol (AKA acetaminophen) is a widely used drug and is used for mild to moderate pains, such as mild osteoarthritis, toothache, headache, and pain caused by minimally invasive surgeries. Despite being a harmless drug in lower doses, acetaminophen can be toxic to the liver and kidneys if overdosed and even results in death. In this study, the therapeutic effects of Solidago canadensis L. extract (SCE) were investigated. 48 adult male Swiss albino mice (20-30 grams) were randomly divided into six groups of 8. The control group was gavaged with normal saline every 12 hours for 6 days. The second group received paracetamol at a 500 mg/kg intraperitoneally (i.p) dose on the sixth day. The third, fourth, and fifth groups were gavaged doses of 125, 250, and 500 mg/kg of SCE every 12 hours for six days, respectively, and on the sixth day, we received paracetamol at a dose of 500 mg/kg i.p. The sixth group only received SCE every 12 hours at a dose of 1000 mg/kg via gavaging for six days. On the seventh day (24 hours after paracetamol injection), blood samples were collected to measure the serum level of creatinine, uric acid, blood urea nitrogen (BUN), total protein, albumin, alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), and total and direct bilirubin, and liver and kidney tissues were also sampled for histopathological examination. It was observed that paracetamol caused a considerable increase in the ALT, AST, ALP, uric Acid, and BUN levels (P < 0.01), while those in SCE-treated groups were significantly lower. In addition, various lesions in the paracetamol group were observed, while in the SCE-receiving groups, receiving prophylactic SCE inhibited the high-intense lesions such as the infiltration of inflammatory cells, hyperemia, and vacuolar degeneration, which decreased significantly in the control group in comparison with that of the paracetamol group (P < 0.05). In conclusion, SCE can have substantial protective effects against paracetamol's hepatorenal toxicity.

Abstract Image

Abstract Image

Abstract Image

加拿大一枝黄花水醇提取物抗扑热息痛毒性小鼠肝肾保护作用。
扑热息痛(又名扑热息痛)是一种广泛使用的药物,用于轻度至中度疼痛,如轻度骨关节炎、牙痛、头痛和微创手术引起的疼痛。尽管对乙酰氨基酚在低剂量下是一种无害的药物,但如果过量服用,它会对肝脏和肾脏产生毒性,甚至导致死亡。本研究对加拿大一枝黄花提取物(SCE)的治疗作用进行了研究。48只成年雄性瑞士白化小鼠(20 ~ 30克),随机分为6组,每组8只。对照组小鼠每隔12 h灌胃生理盐水,连续灌胃6 d。第二组于第6天给予扑热息痛500 mg/kg腹腔注射。第三组、第四组和第五组分别每12小时灌胃125、250和500 mg/kg的SCE,连续6天,第6天给予扑热息痛,剂量为500 mg/kg。第六组每12小时只灌胃SCE,剂量为1000 mg/kg,连续6天。第7天(注射扑热息痛后24小时),取血测定血清肌酐、尿酸、血尿素氮(BUN)、总蛋白、白蛋白、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)、总胆红素和直接胆红素水平,并取肝肾组织进行组织病理学检查。结果显示,扑热息痛引起大鼠血清ALT、AST、ALP、尿酸和BUN水平显著升高(P < 0.01),而sce处理组显著降低。此外,对扑热息痛组观察到各种病变,而在SCE治疗组中,预防性SCE治疗对炎症细胞浸润、充血、空泡变性等高强度病变有抑制作用,与扑热息痛组相比,对照组明显降低(P < 0.05)。综上所述,SCE对扑热息痛的肝肾毒性具有明显的保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Toxicology
Journal of Toxicology TOXICOLOGY-
CiteScore
5.50
自引率
3.40%
发文量
0
审稿时长
10 weeks
期刊介绍: Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences. The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological medicine, risk assessment, safety evaluation, and environmental health.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信