Evaluation of platelet additive solution for prolonging storage of functional canine platelet concentrate

IF 1.1 3区农林科学 Q3 VETERINARY SCIENCES

Journal of veterinary emergency and critical care Pub Date : 2023-08-28 DOI:10.1111/vec.13335

Avin Arjoonsingh DVM, Jillian M. Haines DVM, MS, DACVIM, Julianne K. Hwang PhD, Sarah C. Guess DVM, MS, DACVIM, K. Jane Wardrop DVM, MS, DACVP

{"title":"Evaluation of platelet additive solution for prolonging storage of functional canine platelet concentrate","authors":"Avin Arjoonsingh DVM, Jillian M. Haines DVM, MS, DACVIM, Julianne K. Hwang PhD, Sarah C. Guess DVM, MS, DACVIM, K. Jane Wardrop DVM, MS, DACVP","doi":"10.1111/vec.13335","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>To assess storage lesion development, platelet function, and bacterial growth in canine platelet concentrates (PCs) stored in a platelet additive solution (PAS) or a plasma control at 4°C for 21 days.</p>\n </section>\n \n <section>\n \n <h3> Design</h3>\n \n <p>Prospective, ex vivo, experimental controlled study.</p>\n </section>\n \n <section>\n \n <h3> Setting</h3>\n \n <p>University veterinary teaching hospital.</p>\n </section>\n \n <section>\n \n <h3> Animals</h3>\n \n <p>Ten units of canine PCs collected from blood bank donations.</p>\n </section>\n \n <section>\n \n <h3> Interventions</h3>\n \n <p>The PCs were separated into 2 bags, 1 containing 100% plasma and the other containing 35% plasma and 65% of a PAS (Plasma-Lyte A), and stored at 4°C for 21 days. At days 0, 7, 14, and 21, PCs were analyzed for the presence of swirling, aggregate formation, platelet counts, platelet indices, glucose, lactate, lactate dehydrogenase, Pv<span>co</span><sub>2</sub>, Pv<span>o</span><sub>2</sub>, aggregation via light aggregometry, activation percentages using flow cytometry, and bacterial growth.</p>\n </section>\n \n <section>\n \n <h3> Measurements and main results</h3>\n \n <p>Cold-stored PCs in both PAS and plasma control maintained mean pH >6.8 and mean lactate <9.0 mmol/L over 21 days, with no difference in glucose utilization. Swirl was maintained in both solutions for most days (76/80 combined total samples), with no difference in aggregate formation between solutions. The Pv<span>co</span><sub>2</sub> was higher in plasma on all days (<i>P</i> < 0.001), with no difference in Pv<span>o</span><sub>2</sub>. Platelet indices did not reflect significant storage lesion development in either solution. Lactate dehydrogenase did not differ between solutions but did increase from day 7 to day 21. Mean maximal aggregation percentage was reduced overall but with no significant difference between solutions. The only observed difference in mean activation percentage between solutions was in PAS on day 7, which was significantly higher than plasma (<i>P</i> < 0.05). No bacterial growth occurred during storage.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Cold storage in PAS and plasma allowed PCs to be stored for up to 21 days with minimal storage lesion development, maintenance of platelet function, limited platelet activation, and no bacterial growth within stored bags.</p>\n </section>\n </div>","PeriodicalId":17603,"journal":{"name":"Journal of veterinary emergency and critical care","volume":"33 6","pages":"656-664"},"PeriodicalIF":1.1000,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/vec.13335","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of veterinary emergency and critical care","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/vec.13335","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Objective

To assess storage lesion development, platelet function, and bacterial growth in canine platelet concentrates (PCs) stored in a platelet additive solution (PAS) or a plasma control at 4°C for 21 days.

Design

Prospective, ex vivo, experimental controlled study.

Setting

University veterinary teaching hospital.

Animals

Ten units of canine PCs collected from blood bank donations.

Interventions

The PCs were separated into 2 bags, 1 containing 100% plasma and the other containing 35% plasma and 65% of a PAS (Plasma-Lyte A), and stored at 4°C for 21 days. At days 0, 7, 14, and 21, PCs were analyzed for the presence of swirling, aggregate formation, platelet counts, platelet indices, glucose, lactate, lactate dehydrogenase, Pvco₂, Pvo₂, aggregation via light aggregometry, activation percentages using flow cytometry, and bacterial growth.

Measurements and main results

Cold-stored PCs in both PAS and plasma control maintained mean pH >6.8 and mean lactate <9.0 mmol/L over 21 days, with no difference in glucose utilization. Swirl was maintained in both solutions for most days (76/80 combined total samples), with no difference in aggregate formation between solutions. The Pvco₂ was higher in plasma on all days (P < 0.001), with no difference in Pvo₂. Platelet indices did not reflect significant storage lesion development in either solution. Lactate dehydrogenase did not differ between solutions but did increase from day 7 to day 21. Mean maximal aggregation percentage was reduced overall but with no significant difference between solutions. The only observed difference in mean activation percentage between solutions was in PAS on day 7, which was significantly higher than plasma (P < 0.05). No bacterial growth occurred during storage.

Conclusions

Cold storage in PAS and plasma allowed PCs to be stored for up to 21 days with minimal storage lesion development, maintenance of platelet function, limited platelet activation, and no bacterial growth within stored bags.

Abstract Image

查看原文本刊更多论文

血小板添加液对功能性犬血小板浓缩物延长贮存期的评价。

目的:观察犬血小板浓缩物(PCs)在血小板添加液(PAS)或血浆对照中4℃保存21天后的贮藏损伤发展、血小板功能和细菌生长情况。设计:前瞻性、离体、实验对照研究。单位:大学兽医教学医院。动物:从血库捐赠的犬类pc收集10个单位。干预措施:将PCs分成2袋，1袋含100%血浆，另一袋含35%血浆和65% PAS (plasma - lyte a)， 4℃保存21天。在第0、7、14和21天，分析PCs是否存在旋流、聚集形成、血小板计数、血小板指数、葡萄糖、乳酸、乳酸脱氢酶、Pvco2、Pvo2、光聚集法聚集、流式细胞术激活百分比和细菌生长。测量结果及主要结果:PAS组和血浆对照组冷库pc的平均pH值均维持在6.8以上，血浆中平均乳酸浓度均高于对照组(P < 0.05)。血小板指数在两种溶液中均未反映明显的储存病变发展。乳酸脱氢酶在不同的溶液中没有差异，但在第7天至第21天有所增加。平均最大聚集百分比总体上降低，但解决方案之间没有显著差异。唯一观察到的溶液之间的平均活化百分比差异是PAS在第7天，显著高于血浆(P结论:PAS和血浆中的冷藏使pc可以保存长达21天，最小的储存损伤发展，维持血小板功能，有限的血小板活化，并且在储存袋内没有细菌生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of veterinary emergency and critical care 农林科学-兽医学

CiteScore

2.50

自引率

15.40%

发文量

121

审稿时长

18-36 weeks

期刊介绍： The Journal of Veterinary Emergency and Critical Care’s primary aim is to advance the international clinical standard of care for emergency/critical care patients of all species. The journal’s content is relevant to specialist and non-specialist veterinarians practicing emergency/critical care medicine. The journal achieves it aims by publishing descriptions of unique presentation or management; retrospective and prospective evaluations of prognosis, novel diagnosis, or therapy; translational basic science studies with clinical relevance; in depth reviews of pertinent topics; topical news and letters; and regular themed issues. The journal is the official publication of the Veterinary Emergency and Critical Care Society, the American College of Veterinary Emergency and Critical Care, the European Veterinary Emergency and Critical Care Society, and the European College of Veterinary Emergency and Critical Care. It is a bimonthly publication with international impact and adheres to currently accepted ethical standards.