Virulence, MLST analysis, and antimicrobial resistance of Campylobacter coli isolated from broiler chickens in Tamil Nadu, India.

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Iranian journal of veterinary research Pub Date : 2022-01-01 DOI:10.22099/IJVR.2022.42199.6135

K Gunasekaran, S Vellapandi, M Ananda Chitra, K Kumaragurubaran

{"title":"Virulence, MLST analysis, and antimicrobial resistance of Campylobacter coli isolated from broiler chickens in Tamil Nadu, India.","authors":"K Gunasekaran, S Vellapandi, M Ananda Chitra, K Kumaragurubaran","doi":"10.22099/IJVR.2022.42199.6135","DOIUrl":null,"url":null,"abstract":"Background: Campylobacter species are the zoonotic bacteria and the most common cause of foodborne gastroenteritis around the world. The link between human campylobacteriosis and infected poultry consumption has been well established.Aims: In this study, we aimed to isolate Campylobacter spp. from chicken and characterize them with molecular methods.Methods: Totally, 241 chicken caecal mucosal scrapings were collected from five districts of Tamil Nadu. Bacterial isolation was done by plating on blood-free Campylobacter selective medium with supplements. Campylobacter species were identified by multiplex PCR and Campylobacter coli isolates were tested for 11 virulence genes by PCR. C. coli isolates were typed based on seven housekeeping genes multilocus sequence typing (MLST) scheme. The antimicrobial susceptibility was determined by a microdilution resazurin assay.Results: The prevalence of C. coli and C. jejuni were 14.94% (36/241), and 3.32% (8/241), respectively. The virulence genes flaA, flaB, cadF, cdtA, cdtB, cdtC, ciaB, and ceuE were present in all 36 C. coli isolates, pldA and racR genes were present in 58.33% (21/36), and 16.67% (6/36) of the isolates, respectively, and dnaJ was present in only one isolate. Two novel sequence types (ST-10872, ST-11031) were found in this study. Though different STs were identified, all the STs belonged to the same clonal complex of ST-828. All 14 C. coli isolates showed 100% resistance to nalidixic acid, and higher resistance to tetracycline (92.8%), erythromycin (71.4%), clindamycin (71.4%), and azithromycin (64.2%) was noticed. All C. coli isolates were sensitive to chloramphenicol, and higher sensitivity to ciprofloxacin (78.5%), and gentamicin (71.4%) was observed.Conclusion: The present study demonstrated that C. coli is more prevalent in broilers than C. jejuni in Tamil Nadu. The presence of C. jejuni and C. coli in chicken caecal samples from the slaughterhouse are indicative of the possibility of public health hazards.","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"23 2","pages":"128-136"},"PeriodicalIF":0.8000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9441163/pdf/jvr-23-128.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.22099/IJVR.2022.42199.6135","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Campylobacter species are the zoonotic bacteria and the most common cause of foodborne gastroenteritis around the world. The link between human campylobacteriosis and infected poultry consumption has been well established.

Aims: In this study, we aimed to isolate Campylobacter spp. from chicken and characterize them with molecular methods.

Methods: Totally, 241 chicken caecal mucosal scrapings were collected from five districts of Tamil Nadu. Bacterial isolation was done by plating on blood-free Campylobacter selective medium with supplements. Campylobacter species were identified by multiplex PCR and Campylobacter coli isolates were tested for 11 virulence genes by PCR. C. coli isolates were typed based on seven housekeeping genes multilocus sequence typing (MLST) scheme. The antimicrobial susceptibility was determined by a microdilution resazurin assay.

Results: The prevalence of C. coli and C. jejuni were 14.94% (36/241), and 3.32% (8/241), respectively. The virulence genes flaA, flaB, cadF, cdtA, cdtB, cdtC, ciaB, and ceuE were present in all 36 C. coli isolates, pldA and racR genes were present in 58.33% (21/36), and 16.67% (6/36) of the isolates, respectively, and dnaJ was present in only one isolate. Two novel sequence types (ST-10872, ST-11031) were found in this study. Though different STs were identified, all the STs belonged to the same clonal complex of ST-828. All 14 C. coli isolates showed 100% resistance to nalidixic acid, and higher resistance to tetracycline (92.8%), erythromycin (71.4%), clindamycin (71.4%), and azithromycin (64.2%) was noticed. All C. coli isolates were sensitive to chloramphenicol, and higher sensitivity to ciprofloxacin (78.5%), and gentamicin (71.4%) was observed.

Conclusion: The present study demonstrated that C. coli is more prevalent in broilers than C. jejuni in Tamil Nadu. The presence of C. jejuni and C. coli in chicken caecal samples from the slaughterhouse are indicative of the possibility of public health hazards.

Abstract Image

查看原文本刊更多论文

从印度泰米尔纳德邦肉鸡中分离出的大肠弯曲杆菌的毒性、MLST 分析和抗菌药耐药性。

背景：弯曲杆菌是人畜共患细菌，也是全球食源性胃肠炎最常见的病因。人类弯曲杆菌病与食用受感染家禽之间的联系已经得到证实。目的：在这项研究中，我们旨在从鸡肉中分离出弯曲杆菌属，并用分子方法鉴定其特征：方法：从泰米尔纳德邦的五个地区共收集了 241 份鸡盲肠粘膜刮片。细菌分离是通过在添加补充剂的无血弯曲菌选择性培养基上进行的。通过多重 PCR 鉴定弯曲杆菌的种类，并通过 PCR 检测大肠弯曲杆菌分离物的 11 种毒力基因。根据 7 个看家基因多焦点序列分型（MLST）方案对大肠杆菌分离物进行分型。抗菌药敏感性通过微量稀释法测定：结果：大肠杆菌和空肠杆菌的感染率分别为 14.94%（36/241）和 3.32%（8/241）。所有 36 个大肠杆菌分离物中都存在毒力基因 flaA、flaB、cadF、ctdtA、ctdtB、ctdtC、ciaB 和 ceuE，pldA 和 racR 基因分别存在于 58.33%（21/36）和 16.67%（6/36）的分离物中，而 dnaJ 仅存在于一个分离物中。本研究发现了两种新的序列类型（ST-10872、ST-11031）。虽然发现了不同的 ST，但所有 ST 都属于 ST-828 的同一克隆复合体。所有 14 个大肠杆菌分离物对萘啶酸的耐药性均为 100%，对四环素（92.8%）、红霉素（71.4%）、克林霉素（71.4%）和阿奇霉素（64.2%）的耐药性较高。所有大肠杆菌分离株都对氯霉素敏感，对环丙沙星（78.5%）和庆大霉素（71.4%）的敏感性较高：本研究表明，在泰米尔纳德邦，肉鸡中大肠杆菌的发病率高于空肠杆菌。屠宰场的鸡盲肠样本中存在空肠大肠杆菌和大肠杆菌，这表明可能会对公共健康造成危害。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Iranian journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

8.30%

发文量

期刊介绍： The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.