First substantiation of clinico-molecular investigation of pathogenic Listeria monocytogenes in Nili-Ravi buffaloes.

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Iranian journal of veterinary research Pub Date : 2022-01-01 DOI:10.22099/IJVR.2022.41671.6062

M Z Munir, J A Khan, M Ijaz, F Akhtar

{"title":"First substantiation of clinico-molecular investigation of pathogenic Listeria monocytogenes in Nili-Ravi buffaloes.","authors":"M Z Munir, J A Khan, M Ijaz, F Akhtar","doi":"10.22099/IJVR.2022.41671.6062","DOIUrl":null,"url":null,"abstract":"Background: Listeriosis is a zoonotic disease of humans, animals, birds, fish, and crustaceans worldwide. Domestic animals, especially ruminants, are more susceptible to listeriosis. This infectious disease is caused by Listeria monocytogenes, an intracellular bacterium that can cross blood-brain, placental and intestinal barriers. In Pakistan, the incidence and reliable diagnostic tools for the L. monocytogenes are unidentified in Nili-Ravi buffaloes.Aims: This study was designed to inspect listeriosis in buffaloes through molecular techniques and haemato-biochemical analyses.Methods: A total of 230 samples (115 milk and 115 faecal samples) were collected from symptomatic listeriosis cases in Nili-Ravi buffaloes of 3 geographical districts (Rawalpindi, Faisalabad, and Muzaffargarh) Punjab, Pakistan. These samples were processed for DNA extraction using commercialized kits, and L. monocytogenes was confirmed by conventional PCR.Results: The results revealed that 6.08% and 4.34% of the isolates from milk and faecal samples were found positive for L. monocytogenes, respectively. The phylogenetic analysis of these isolates showed 97-100% similarity to isolates from the USA, Switzerland, Japan, and India. The accession numbers on NCBI GenBank appeared as HF558398 (Switzerland), KP965732 (India), EU372032 (USA), and LC259850 (Japan). Haemato-biochemical examinations showed that the values of WBCs, plasma fibrinogen, ALT, and AST significantly increased (P<0.05) in diseased buffaloes compared to healthy ones.Conclusion: The occurrence of listeriosis in buffaloes urges continuous monitoring and surveillance to prevent this emerging disease in Pakistan.","PeriodicalId":14629,"journal":{"name":"Iranian journal of veterinary research","volume":"23 2","pages":"120-127"},"PeriodicalIF":0.8000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9441160/pdf/jvr-23-120.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.22099/IJVR.2022.41671.6062","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Listeriosis is a zoonotic disease of humans, animals, birds, fish, and crustaceans worldwide. Domestic animals, especially ruminants, are more susceptible to listeriosis. This infectious disease is caused by Listeria monocytogenes, an intracellular bacterium that can cross blood-brain, placental and intestinal barriers. In Pakistan, the incidence and reliable diagnostic tools for the L. monocytogenes are unidentified in Nili-Ravi buffaloes.

Aims: This study was designed to inspect listeriosis in buffaloes through molecular techniques and haemato-biochemical analyses.

Methods: A total of 230 samples (115 milk and 115 faecal samples) were collected from symptomatic listeriosis cases in Nili-Ravi buffaloes of 3 geographical districts (Rawalpindi, Faisalabad, and Muzaffargarh) Punjab, Pakistan. These samples were processed for DNA extraction using commercialized kits, and L. monocytogenes was confirmed by conventional PCR.

Results: The results revealed that 6.08% and 4.34% of the isolates from milk and faecal samples were found positive for L. monocytogenes, respectively. The phylogenetic analysis of these isolates showed 97-100% similarity to isolates from the USA, Switzerland, Japan, and India. The accession numbers on NCBI GenBank appeared as HF558398 (Switzerland), KP965732 (India), EU372032 (USA), and LC259850 (Japan). Haemato-biochemical examinations showed that the values of WBCs, plasma fibrinogen, ALT, and AST significantly increased (P<0.05) in diseased buffaloes compared to healthy ones.

Conclusion: The occurrence of listeriosis in buffaloes urges continuous monitoring and surveillance to prevent this emerging disease in Pakistan.

Abstract Image

查看原文本刊更多论文

首次证实尼利-拉维水牛致病性李斯特菌的临床-分子研究。

背景：李斯特菌病是一种人畜共患病，世界各地的人类、动物、鸟类、鱼类和甲壳类都可能感染。家畜，尤其是反刍动物更容易感染李斯特菌病。这种传染病由李斯特菌引起，李斯特菌是一种细胞内细菌，可以穿过血脑屏障、胎盘屏障和肠道屏障。目的：本研究旨在通过分子技术和血液生化分析检测水牛的李斯特菌病：从巴基斯坦旁遮普省 3 个地区（拉瓦尔品第、费萨拉巴德和穆扎法尔加尔）有症状的尼利-拉维水牛中收集了 230 份样本（115 份牛奶样本和 115 份粪便样本）。使用商品化试剂盒对这些样本进行了 DNA 提取处理，并通过传统 PCR 方法确认了单核细胞增多症：结果显示，牛奶和粪便样本中分别有 6.08% 和 4.34% 的分离物对单核细胞增多性酵母菌呈阳性。对这些分离物的系统进化分析表明，它们与来自美国、瑞士、日本和印度的分离物的相似度为 97%-100%。在 NCBI GenBank 上的登录号分别为 HF558398（瑞士）、KP965732（印度）、EU372032（美国）和 LC259850（日本）。血液生化检查显示，白细胞、血浆纤维蛋白原、谷丙转氨酶和谷草转氨酶的数值明显升高（PC结论：水牛李斯特菌病的发生要求对其进行持续监测和监控，以防止这种新疾病在巴基斯坦出现。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Iranian journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

8.30%

发文量

期刊介绍： The Iranian Journal of Veterinary Research(IJVR) is published quarterly in 4 issues. The aims of this journal are to improve and expand knowledge in all veterinary fields. It is an international journal indexed by the Thomson Institute for Scientific Information (ISI), Elsevier, Scopus, CAB International, Veterinary Bulletin and several other international databases. Research papers and reports on a wide range of veterinary topics are published in the journal after being evaluated by expert reviewers.The Editor-in-Chief is responsible for the editorial content of the journal—including peer-reviewed manuscripts—and the timing of its publication.