Investigation of Peptide Labeling with ortho-Phthalaldehyde and 2-Acylbenzaldehyde.

IF 3.3 2区化学 Q1 CHEMISTRY, ORGANIC

The Journal of Organic Chemistry Pub Date : 2024-10-18 Epub Date: 2023-08-22 DOI:10.1021/acs.joc.3c01397

Fan Xiao, Mengze Sun, Liyun Zhang, Xiaoguang Lei

引用次数: 0

Abstract

ortho-Phthalaldehyde (OPA) with high reactivity to the amine group has been widely used to modify proteins. We discovered new modifications of OPA and 2-acylbenzaldehyde and proposed the reaction mechanism. Using isotope labeling mass spectrometry-based experiment, we identified new cross-linking properties of OPA and 2-acylbenzaldehyde. This reactivity revealed that OPA has the potential to probe proximal amino acids in biological systems.

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用邻苯二甲醛和 2-酰基苯甲醛标记多肽的研究。

邻苯二甲醛（OPA）对胺基具有高反应活性，已被广泛用于修饰蛋白质。我们发现了 OPA 和 2-酰基苯甲醛的新修饰作用，并提出了反应机理。通过同位素标记质谱实验，我们发现了 OPA 和 2-酰基苯甲醛新的交联特性。这种反应性揭示了 OPA 具有探测生物系统中近端氨基酸的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

The Journal of Organic Chemistry 化学-有机化学

CiteScore

6.20

自引率

11.10%

发文量

1467

审稿时长

2 months

期刊介绍： The Journal of Organic Chemistry welcomes original contributions of fundamental research in all branches of the theory and practice of organic chemistry. In selecting manuscripts for publication, the editors place emphasis on the quality and novelty of the work, as well as the breadth of interest to the organic chemistry community.