Bioprospecting and Diversity of Yeast Producing Ethanol Isolated from Indonesia.

IF 1.1 Q3 BIOLOGY

Tropical life sciences research Pub Date : 2022-09-01 DOI:10.21315/tlsr2022.33.3.1

Eny Ida Riyanti, Rafika Yuniawati, Edy Listanto

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引用次数: 1

Abstract

Bioethanol is considered the most environmentally friendly as renewable fuels. Indonesia has abundant microbe diversity which is potential for bioprospecting such as fermenting agents using agriculture product as raw materials for producing bioethanol. This study aims to isolate, characterise and molecular identify of 15 isolates of bioethanol-producing yeasts from various sources. Characterisation based on ethanol production, cell morphology and various substrate utilisation has been carried out. Molecular characterisation of 15 yeast isolates using tree sets of primers had been carried out. Amplification in the internal area of transcribe spacers (ITS) was successfully carried out with an amplitude of 400 bp-900 bp. Amplifiers in the D1/D2 26s rDNA domain are 250 bp. Amplification with ScerF2 and ScerR2 specific primers was carried out successfully and proved that there were two isolates which were not Saccharomyces cerevisiae analysis of yeast genetic diversity showed 12 yeast isolates classified as S. cerevisiae and the rest belonged to the genus Clavispora, Candida and Kodamaea (Pichia).

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印度尼西亚产乙醇酵母菌的生物勘探及其多样性。

生物乙醇被认为是最环保的可再生燃料。印尼拥有丰富的微生物多样性，具有利用农产品作为原料生产生物乙醇的发酵剂等生物勘探潜力。本研究旨在分离、表征和分子鉴定来自不同来源的15株产乙醇酵母菌。基于乙醇生产，细胞形态和各种底物利用的特性已经进行了。利用3组引物对15株酵母菌分离株进行了分子鉴定。转录间隔片段(ITS)的内部区域扩增成功，扩增幅度为400bp - 900bp。D1/D2 26s rDNA结构域的放大器为250bp。用ScerF2和ScerR2特异性引物进行扩增，证实其中有2株分离物不是酿酒酵母，酵母遗传多样性分析显示12株分离物属于酿酒酵母，其余分别属于Clavispora属、Candida属和Kodamaea (Pichia)属。

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来源期刊

Tropical life sciences research BIOLOGY-

CiteScore

2.60

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： Tropical Life Sciences Research (TLSR) formerly known as Journal of Bioscience seeks to publish relevant ideas and knowledge addressing vital life sciences issues in the tropical region. The Journal’s scope is interdisciplinary in nature and covers any aspects related to issues on life sciences especially from the field of biochemistry, microbiology, biotechnology and animal, plant, environmental, biomedical and pharmaceutical sciences. TLSR practices double blind peer review system to ensure and maintain the good quality of articles published in this journal. Two issues are published annually in printed and electronic form. TLSR also accepts review articles, experimental papers and short communications. The Chief Editor would like to invite researchers to use this journal as a mean to rapidly promote their research findings.