In vivo investigation of open-pored magnesium scaffolds LAE442 with different coatings in an open wedge defect.

IF 3.1 4区 医学 Q2 BIOPHYSICS
Marlene Schmidt, Anja-Christina Waselau, Franziska Feichtner, Stefan Julmi, Christian Klose, Hans Jürgen Maier, Peter Wriggers, Andrea Meyer-Lindenberg
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引用次数: 0

Abstract

The magnesium alloy LAE442 showed promising results as a bone substitute in numerous studies in non-weight bearing bone defects. This study aimed to investigate the in vivo behavior of wedge-shaped open-pored LAE442 scaffolds modified with two different coatings (magnesium fluoride (MgF2, group 1)) or magnesium fluoride/calcium phosphate (MgF2/CaP, group 2)) in a partial weight-bearing rabbit tibia defect model. The implantation of the scaffolds was performed as an open wedge corrective osteotomy in the tibia of 40 rabbits and followed for observation periods of 6, 12, 24, and 36 weeks. Radiological and microcomputed tomographic examinations were performed in vivo. X-ray microscopic, histological, histomorphometric, and SEM/EDS analyses were performed at the end of each time period. µCT measurements and X-ray microscopy showed a slight decrease in volume and density of the scaffolds of both coatings. Histologically, endosteal and periosteal callus formation with good bridging and stabilization of the osteotomy gap and ingrowth of bone into the scaffold was seen. The MgF2 coating favored better bridging of the osteotomy gap and more bone-scaffold contacts, especially at later examination time points. Overall, the scaffolds of both coatings met the requirement to withstand the loads after an open wedge corrective osteotomy of the proximal rabbit tibia. However, in addition to the inhomogeneous degradation behavior of individual scaffolds, an accumulation of gas appeared, so the scaffold material should be revised again regarding size dimension and composition.

不同涂层的开孔镁支架LAE442在开放性楔形缺陷中的体内研究。
LAE442镁合金作为骨替代物在非负重骨缺损的大量研究中显示出良好的效果。本研究旨在研究两种不同涂层(氟化镁(MgF2,第1组)或氟化镁/磷酸钙(MgF2/CaP,第2组)修饰的楔形开孔LAE442支架在兔部分负重胫骨缺损模型中的体内行为。40只家兔胫骨采用开放楔形矫正截骨术植入支架,随访观察6、12、24、36周。在体内进行放射学和显微计算机断层扫描检查。在每个时间段结束时进行x射线显微镜、组织学、组织形态学和扫描电镜/能谱分析。微CT测量和x射线显微镜显示两种涂层支架的体积和密度略有下降。组织学上,骨内和骨膜骨痂形成,截骨间隙良好的桥接和稳定,骨长入支架。MgF2涂层有利于更好地桥接截骨间隙和更多的骨支架接触,特别是在较晚的检查时间点。总的来说,两种涂层的支架都满足了兔胫骨近端开放楔形矫正截骨后的负荷要求。然而,除了单个支架的不均匀降解行为外,还出现了气体的积聚,因此支架材料需要在尺寸、尺寸和成分上再次进行修改。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Applied Biomaterials & Functional Materials
Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL
CiteScore
4.40
自引率
4.00%
发文量
36
审稿时长
>12 weeks
期刊介绍: The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics
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