Core genome multilocus sequence typing scheme for Bacillus cereus group bacteria

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology Pub Date : 2023-07-01 DOI:10.1016/j.resmic.2023.104050

Nicolas J. Tourasse , Keith A. Jolley , Anne-Brit Kolstø , Ole Andreas Økstad

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引用次数: 2

Abstract

Core genome multilocus sequence typing (cgMLST) employs a strategy where the set of orthologous genes common to all members of a group of organisms are used for phylogenetic analysis of the group members. The Bacillus cereus group consists of species with pathogenicity towards insect species as well as warm-blooded animals including humans. While B. cereus is an opportunistic pathogen linked to a range of human disease conditions, including emesis and diarrhoea, Bacillus thuringiensis is an entomopathogenic species with toxicity toward insect larvae, and therefore used as a biological pesticide worldwide. Bacillus anthracis is a classical obligate pathogen causing anthrax, an acute lethal condition in herbivores as well as humans, and which is endemic in many parts of the world. The group also includes a range of additional species, and B. cereus group bacteria have been subject to analysis with a wide variety of phylogenetic typing systems. Here we present, based on analyses of 173 complete genomes from B. cereus group species available in public databases, the identification of a set of 1568 core genes which were used to create a core genome multilocus typing scheme for the group which is implemented in the PubMLST system as an open online database freely available to the community. The new cgMLST system provides unprecedented resolution over existing phylogenetic analysis schemes covering the B. cereus group.

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蜡样芽孢杆菌群细菌核心基因组多位点序列分型方案

核心基因组多点序列分型（cgMLST）采用了一种策略，其中使用一组生物的所有成员共有的一组同源基因来对组成员进行系统发育分析。蜡样芽孢杆菌群包括对昆虫和包括人类在内的温血动物具有致病性的物种。蜡状芽孢杆菌是一种机会性病原体，与包括呕吐和腹泻在内的一系列人类疾病有关，而苏云金芽孢杆菌是一个对昆虫幼虫具有毒性的昆虫病原物种，因此在世界范围内被用作生物农药。炭疽杆菌是一种典型的专性病原体，引起炭疽病，炭疽病是食草动物和人类的急性致命疾病，在世界许多地区流行。该类群还包括一系列其他物种，蜡状芽孢杆菌类群细菌已通过各种系统发育分型系统进行了分析。在这里，我们基于对公共数据库中可用的蜡状芽孢杆菌群物种的173个完整基因组的分析，鉴定了一组1568个核心基因，这些基因用于创建该群的核心基因组多位点分型方案，该方案在PubMLST系统中作为一个开放的在线数据库免费提供给社区。新的cgMLST系统比覆盖蜡状芽孢杆菌群的现有系统发育分析方案提供了前所未有的分辨率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in microbiology 生物-微生物学

CiteScore

4.10

自引率

3.80%

发文量

审稿时长

16 days

期刊介绍： Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.