Human Hepatocellular response in Cholestatic Liver Diseases.

IF 1.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Organogenesis Pub Date : 2023-12-31 DOI:10.1080/15476278.2023.2247576

Kimberly Ortiz, Zeliha Cetin, Yiyue Sun, Zhiping Hu, Takeshi Kurihara, Edgar N Tafaleng, Rodrigo M Florentino, Alina Ostrowska, Alejandro Soto-Gutierrez, Lanuza A P Faccioli

引用次数: 0

Abstract

Primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC), the most common types of cholestatic liver disease (CLD), result in enterohepatic obstruction, bile acid accumulation, and hepatotoxicity. The mechanisms by which hepatocytes respond to and cope with CLD remain largely unexplored. This study includes the characterization of hepatocytes isolated from explanted livers of patients with PBC and PSC. We examined the expression of hepatocyte-specific genes, intracellular bile acid (BA) levels, and oxidative stress in primary-human-hepatocytes (PHHs) isolated from explanted livers of patients with PBC and PSC and compared them with control normal human hepatocytes. Our findings provide valuable initial insights into the hepatocellular response to cholestasis in CLD and help support the use of PHHs as an experimental tool for these diseases.

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胆固醇性肝病中的人肝细胞反应。

原发性胆汁性胆管炎（PBC）和原发性硬化性胆管管炎（PSC）是最常见的胆汁淤积性肝病（CLD）类型，可导致肠肝梗阻、胆汁酸积聚和肝毒性。肝细胞对CLD反应和应对CLD的机制在很大程度上尚未探索。本研究包括从PBC和PSC患者的移植肝脏中分离的肝细胞的特征。我们检测了从PBC和PSC患者的移植肝脏中分离的原代人肝细胞（PHH）中肝细胞特异性基因的表达、细胞内胆汁酸（BA）水平和氧化应激，并将其与对照正常人肝细胞进行比较。我们的发现为CLD中肝细胞对胆汁淤积的反应提供了有价值的初步见解，并有助于支持PHHs作为治疗这些疾病的实验工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY

CiteScore

4.10

自引率

4.30%

发文量

审稿时长

>12 weeks

期刊介绍： Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.