Morinda officinalis polysaccharide attenuates osteoporosis in rats underwent bilateral ovariectomy by suppressing the PGC-1α/PPARγ pathway.

IF 1.3 4区医学 Q3 ORTHOPEDICS

Journal of Orthopaedic Surgery Pub Date : 2022-09-01 DOI:10.1177/10225536221130824

Kai Rong, Pingbo Chen, Yi Lang, Yaowu Zhang, Zhan Wang, Fengli Wen, Laijin Lu

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引用次数: 1

Abstract

Objective: Osteoporosis (OP) is a widespread disease that causes risks of spine and hip fractures. Morinda officinalis polysaccharide (MOP) shows therapeutic potential in OP. This article intended to understand the mechanism by which MOP impacts bone mineral density (BMD) and serum trace elements in OP rats.

Methods: OP rat models were established by bilateral ovariectomy (OVX). Rats were intragastrically administered with MOP or ZLN005 [the activator of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α)] since the first day after operation for 8 weeks. Microstructural changes in OP rats were analyzed using micro-computed tomography system. Contents of serum Zn, Cu, Fe, and Mg in rats were measured. Levels of serum superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), GSH, and malondialdehyde (MDA) in rats were determined by Enzyme-linked immunosorbent assay. Protein levels of PGC-1α and peroxisome proliferator-activated receptor γ (PPARγ) in cartilage tissues of rats were determined via Western blotting.

Results: MOP enhanced BMD, bone volume per trabecular volume (BV/TV), Tb.N, and Tb.Th and reduced Tb.Sp in the distal femur of OVX rats, elevated levels of serum Cu, Fe, and Mg and contents of SOD, GSH, and GSH-PX and decreased MDA content. Moreover, MOP suppressed the PGC-1α/PPARγ pathway. Activation of PGC-1α partially abolished the action of MOP on ameliorating OP in OVX rats and strengthening anti-oxidation ability.

Conclusion: MOP mitigated OP in OVX rats by inhibiting the PGC-1α/PPARγ pathway.

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巴桑多糖通过抑制PGC-1α/PPARγ通路减轻双侧卵巢切除术大鼠骨质疏松症。

目的:骨质疏松症(OP)是一种广泛存在的疾病，可导致脊柱和髋部骨折的危险。巴戟多糖(Morinda officinalis多糖，MOP)对OP大鼠骨矿物质密度(BMD)和血清微量元素的影响机制。方法:采用双侧卵巢切除术(OVX)建立OP大鼠模型。术后第1天大鼠ig MOP或ZLN005(过氧化物酶体增殖物激活受体-γ共激活因子-1α (PGC-1α)激活剂)，持续8周。应用显微计算机断层扫描系统分析OP大鼠的微结构变化。测定大鼠血清锌、铜、铁、镁的含量。采用酶联免疫吸附法测定大鼠血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH- px)、谷胱甘肽过氧化物酶(GSH)和丙二醛(MDA)水平。Western blotting检测大鼠软骨组织中PGC-1α和过氧化物酶体增殖物激活受体γ (PPARγ)蛋白水平。结果:MOP可提高骨密度，骨体积/小梁体积(BV/TV)， Tb。N和Tb。减小了Tb。OVX大鼠股骨远端Sp含量升高，血清Cu、Fe、Mg水平升高，SOD、GSH、GSH- px含量升高，MDA含量降低。此外，MOP抑制PGC-1α/PPARγ通路。PGC-1α的激活部分取消了MOP改善OVX大鼠OP和增强抗氧化能力的作用。结论:MOP通过抑制PGC-1α/PPARγ通路减轻OVX大鼠OP。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Orthopaedic Surgery ORTHOPEDICS-SURGERY

CiteScore

3.10

自引率

0.00%

发文量

审稿时长

13 weeks

期刊介绍： Journal of Orthopaedic Surgery is an open access peer-reviewed journal publishing original reviews and research articles on all aspects of orthopaedic surgery. It is the official journal of the Asia Pacific Orthopaedic Association. The journal welcomes and will publish materials of a diverse nature, from basic science research to clinical trials and surgical techniques. The journal encourages contributions from all parts of the world, but special emphasis is given to research of particular relevance to the Asia Pacific region.