Up-regulation of HSPA1A and HSPA1B in the blood of tophi patients and its clinical significance.

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Acta biochimica Polonica Pub Date : 2022-12-14 DOI:10.18388/abp.2020_6066

Yang Li, Chen Shan, Bo Yang, Hu Wang

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引用次数: 0

Abstract

Objective: Poorly treated gout can cause tophi, which can lead to serious and potentially fatal complications. This study aimed to find the potential diagnostic value of blood levels of HSPA1A and HSPA1B for tophi patients.

Methods: 58 tophi patients and 61 healthy controls were enrolled in this study, and the whole venous blood samples of all subjects were collected for microarray analysis to identify differentially expressed genes associated with tophi. Meanwhile, KEGG and GO analysis were used to filtrate the enriched different expression genes. The mRNA expression levels of HSPA1A, as well as HSPA1B, were measured by the RT-qPCR method, the correlation between which and the severity of the disease were analyzed. Finally, the receiver operating characteristics curve (ROC) analysis has been performed to the diagnostic value of HSPA1A as well as HSPA1B.

Results: Bioinformatic analysis results suggested that both HSPA1A and HSPA1B are abnormally expressed in tophi. Then, it was observed that HSPA1A and HSPA1B were dramatically increased in the blood samples of tophi patients compared with healthy controls and were further linked with the severity of tophi. Moreover, the area under the curve (AUC) of HSPA1A for the diagnosis of ACI was 0.8999 (95% confidence interval (CI), 0.8338 to 0.9661) while of HSPA1B was 0.9093 (95% confidence interval (CI), 0.8550 to 0.9635), suggesting that blood level of HSPA1A, as well as HSPA1B, are sensitive markers to distinguish tophi patients from the healthy people.

Conclusion: HSPA1A and HSPA1B were over-expressed in the blood of tophi patients and may be potential diagnostic markers for tophi.

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痛风患者血中HSPA1A和HSPA1B的上调及其临床意义。

目的:治疗不当的痛风可引起痛风，痛风可导致严重和潜在致命的并发症。本研究旨在探讨血中HSPA1A和HSPA1B水平对痛风患者的潜在诊断价值。方法:选取58例痛风患者和61例健康对照者，采集全静脉血进行微阵列分析，鉴定痛风相关差异表达基因。同时，采用KEGG和GO分析对富集的不同表达基因进行筛选。RT-qPCR法检测HSPA1A、HSPA1B mRNA表达水平，分析其与疾病严重程度的相关性。最后，对HSPA1A和HSPA1B的诊断价值进行受试者工作特征曲线(ROC)分析。结果:生物信息学分析结果显示，HSPA1A和HSPA1B在痛风石中均有异常表达。然后，我们观察到，与健康对照组相比，痛风患者血液样本中的HSPA1A和HSPA1B显著升高，并进一步与痛风的严重程度相关。HSPA1A诊断ACI的曲线下面积(AUC)为0.8999(95%可信区间(CI) 0.8338 ~ 0.9661)， HSPA1B诊断ACI的曲线下面积(AUC)为0.9093(95%可信区间(CI) 0.8550 ~ 0.9635)，提示血中HSPA1A及HSPA1B水平是区分痛风患者与健康人的敏感指标。结论:HSPA1A和HSPA1B在痛风患者血液中过表达，可能是痛风的潜在诊断标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta biochimica Polonica 生物-生化与分子生物学

CiteScore

2.40

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Acta Biochimica Polonica is a journal covering enzymology and metabolism, membranes and bioenergetics, gene structure and expression, protein, nucleic acid and carbohydrate structure and metabolism.