Decreased in n-3 DHA enriched triacylglycerol in small extracellular vesicles of diabetic patients with cardiac dysfunction.

IF 3 2区医学 Q2 ENDOCRINOLOGY & METABOLISM

Journal of Diabetes Pub Date : 2023-12-01 Epub Date: 2023-08-18 DOI:10.1111/1753-0407.13457

Wei Ding, Xuejuan Zhang, Dandan Xiao, Wenguang Chang

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引用次数: 2

Abstract

Purpose: Diabetic cardiomyopathy is the leading cause of death in diabetic patients, and the mechanism by which factors other than hyperglycemia contribute to the development of diabetic cardiomyopathy is unknown. Serum small extracellular vesicles (sEVs) carry bioactive proteins or nuclei, which enter into remote tissues and modulate cell functions. However, in diabetic conditions, the changes of lipids carried by sEVs has not been identified. Our study aims to explore the changes of lipids in sEVs in diabetic patients with cardiovascular disease, we hope to provide new ideas for understanding the role of lipid metabolism in the pathogenesis of diabetic cardiomyopathy.

Methods: SEVs samples derived from serum of health controls (Ctrl), diabetic patients without cardiovascular diseases (DM), and diabetic patients with cardiovascular diseases (DM-CAD) were used for lipidomics analysis. Because AC16 cells are also treated with those sEVs to confirm the entrance of cells and effects on insulin sensitivity, a lipidomics analysis on cells was also performed.

Results and conclusions: In this study, we found that docosahexaenoic acid (DHA)-triacylglycerides of sEVs from serums of DM-CAD patients decreased significantly, and those sEVs could enter into AC16 cells and diminish insulin sensitivity. In addition, DHA-triacylglycerides were also decreased in cells treated with sEVs from DM-CAD. Therefore, DHA-triacylglycerides carried by sEVs may mediate intercellular signaling and be associated with the incidence of diabetic cardiovascular complications.

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心功能不全的糖尿病患者细胞外小泡中富含 n-3 DHA 的三酰甘油减少。

目的：糖尿病心肌病是糖尿病患者死亡的主要原因，而高血糖以外的其他因素导致糖尿病心肌病发生的机制尚不清楚。血清小细胞外囊泡（sEVs）携带生物活性蛋白或细胞核，可进入远端组织并调节细胞功能。然而，在糖尿病条件下，sEVs 所携带的脂质的变化尚未被发现。我们的研究旨在探讨糖尿病心血管疾病患者 sEVs 中脂质的变化，希望能为了解脂质代谢在糖尿病心肌病发病机制中的作用提供新的思路：方法：采用健康对照组（Ctrl）、无心血管疾病的糖尿病患者（DM）和有心血管疾病的糖尿病患者（DM-CAD）血清中的SEVs样本进行脂质组学分析。由于 AC16 细胞也接受了这些 sEVs 的处理，以确认细胞的进入和对胰岛素敏感性的影响，因此也对细胞进行了脂质组学分析：本研究发现，DM-CAD 患者血清中的 sEVs 中二十二碳六烯酸（DHA）-三酰甘油显著减少，这些 sEVs 可以进入 AC16 细胞并降低胰岛素敏感性。此外，用DM-CAD患者血清中的SEVs处理的细胞中DHA-三酰甘油也减少了。因此，sEVs携带的DHA-三酰甘油可能会介导细胞间信号转导，并与糖尿病心血管并发症的发生有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Diabetes ENDOCRINOLOGY & METABOLISM-

CiteScore

6.50

自引率

2.20%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Diabetes (JDB) devotes itself to diabetes research, therapeutics, and education. It aims to involve researchers and practitioners in a dialogue between East and West via all aspects of epidemiology, etiology, pathogenesis, management, complications and prevention of diabetes, including the molecular, biochemical, and physiological aspects of diabetes. The Editorial team is international with a unique mix of Asian and Western participation. The Editors welcome submissions in form of original research articles, images, novel case reports and correspondence, and will solicit reviews, point-counterpoint, commentaries, editorials, news highlights, and educational content.