Reversal of itraconazole resistance in Sporothrix brasiliensis and Sporothrix schenckii by nonsteroidal anti-inflammatory drugs

IF 1.5 4区生物学 Q4 MYCOLOGY

Revista Iberoamericana De Micologia Pub Date : 2022-07-01 DOI:10.1016/j.riam.2022.01.003

Gabriela Seibert , Ana Luisa Reetz Poletto , Jennifer Veppo Prade , Débora Nunes Mario , Cheila Denise Ottonelli Stopiglia

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引用次数: 1

Abstract

Background

The emergence of resistant isolates has brought challenges to the treatment of sporotrichosis, prompting the search for new therapeutic strategies. Previous studies reported that nonsteroidal anti-inflammatory drugs (NSAIDs) show in vitro activity against several pathogenic fungi, including species of Candida, Cryptococcus, and Trichosporon.

Aims

This study aimed to investigate the in vitro efficacy of three NSAIDs (acetylsalicylic acid, diclofenac sodium, and ibuprofen), alone and in combination with itraconazole, against eleven clinical isolates of Sporothrix brasiliensis and Sporothrix schenckii.

Methods

Minimal inhibitory concentrations were determined by the broth microdilution method. Drug interactions and the fractional inhibitory concentration index of NSAIDs and itraconazole were assessed by the checkerboard method.

Results

When used alone, ibuprofen was the most active NSAID, followed by acetylsalicylic acid. Combinations of NSAIDs with itraconazole showed synergistic antifungal activity against nine isolates. It was also found that itraconazole combined with acetylsalicylic acid, diclofenac sodium, or ibuprofen, led to resistance reversal in two, three, and five of the six drug-resistant isolates, respectively.

Conclusions

The results indicate that the combination of itraconazole and the evaluated NSAIDs are a promising strategy for the treatment of sporotrichosis.

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非甾体抗炎药逆转巴西孢子菌和申克孢子菌对伊曲康唑的耐药性

耐药菌株的出现给孢子菌病的治疗带来了挑战，促使人们寻找新的治疗策略。先前的研究报道，非甾体抗炎药(NSAIDs)显示出对几种病原真菌的体外活性，包括念珠菌、隐球菌和毛孢子菌。目的观察3种非甾体抗炎药(乙酰水杨酸、双氯芬酸钠和布洛芬)单独和联合伊曲康唑对11株巴西孢子丝菌和申克孢子丝菌的体外抑菌效果。方法采用微量肉汤稀释法测定最小抑菌浓度。采用棋盘法评价非甾体抗炎药与伊曲康唑的药物相互作用及分数抑制浓度指数。结果单独使用时，布洛芬是最有效的非甾体抗炎药，其次是乙酰水杨酸。非甾体抗炎药与伊曲康唑联用对9株菌株均有协同抗真菌活性。研究还发现，伊曲康唑与乙酰水杨酸、双氯芬酸钠或布洛芬联用后，6株耐药菌株中分别有2株、3株和5株出现耐药性逆转。结论伊曲康唑与评价的非甾体抗炎药联用治疗孢子菌病是一种有前景的治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Revista Iberoamericana De Micologia MYCOLOGY-

CiteScore

1.90

自引率

0.00%

发文量

审稿时长

81 days

期刊介绍： Revista Iberoamericana de Micología (Ibero-American Journal of Mycology) is the official journal of the Asociación Española de Micología, Asociación Venezolana de Micología and Asociación Argentina de Micología (The Spanish, Venezuelan, and Argentinian Mycology Associations). The Journal gives priority to publishing articles on studies associated with fungi and their pathogenic action on humans and animals, as well as any scientific studies on any aspect of mycology. The Journal also publishes, in Spanish and in English, original articles, reviews, mycology forums, editorials, special articles, notes, and letters to the editor, that have previously gone through a scientific peer review process.