Momordica charantia Enhances Tendon Healing in Rats: An Experimental Study.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2024-01-01 Epub Date: 2023-08-16 DOI:10.1159/000533644
Furkan Erdoğan, Arife Ahsen Kaplan, Hüseyin Sina Coşkun, Gamze Altun, Berrin Zuhal Altunkaynak, Ebru Kelsaka, Suleyman Kaplan, Ahmet Pişkin
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Abstract

Momordica charantia (MC) is a traditional plant widely used since ancient times for wound healing. This study evaluated its potential effects on tendon healing. Adult male Wistar albino rats (n = 32, 8 rats in each group) were anesthetized, and their Achilles tendons were prepared for surgical procedures. Group 1 (Cont = control group) was not subjected to any surgery and was used as a control group for baseline values. Group 2 (PR = primary repair group) underwent primary repair (PR) with a monofilament suture after a full-thickness incision of the Achilles tendon. A full-thickness incision was also made to the Achilles tendon of group 3 (CT = collagen tube-administered group), followed by PR and collagen tube insertion. In group 4 (MC = M. charantia-administered group), 1 mL of MC extract was applied locally on the collagen tube in addition to the surgical procedure applied to group 3. The Achilles tendons were excised on the postoperative 40th day and examined stereologically, histologically, and bioinformatically. Data showed that the total volume of the collagen fibers was higher in MC and CT groups than in the PR group. The total volume of the tendon was decreased in MC and CT groups than in the Cont group. The ratios between the volumes of the collagen fibers and total tendon in the MC and CT groups were significantly different from PR, but not different from the Cont group. Additionally, MC improved tenoblastic activity, collagen production, and neovascularization. Bioinformatic interactions showed that the proteases of MC could trigger the signals playing a role on vasculogenesis, reducing inflammation, and contributing to tenoblast activation and collagen remodeling. MC extract ameliorates the healing of injured tendon and can provide satisfactory tendon repair. Further works are recommended to explore the healing capacity of MC.

Momordica charantia Enhances Tendon Healing in Rats:一项实验研究。
Momordica charantia(MC)是一种自古以来被广泛用于伤口愈合的传统植物。本研究评估了它对肌腱愈合的潜在影响。成年雄性 Wistar 白化大鼠(n = 32,每组 8 只)被麻醉后,跟腱准备接受手术治疗。第 1 组(Cont = 对照组)未进行任何手术,作为基线值对照组。第 2 组(PR = 初级修复组)在跟腱全厚切口后用单丝缝合进行初级修复(PR)。第 3 组(CT = 使用胶原管组)也对跟腱进行了全厚切口,然后进行 PR 和胶原管插入。术后第 40 天切除跟腱,并进行立体学、组织学和生物信息学检查。数据显示,MC 组和 CT 组的胶原纤维总体积高于 PR 组。MC组和CT组肌腱的总体积比Cont组减少。MC 组和 CT 组的胶原纤维体积与肌腱总体积之比明显不同于 PR 组,但与对照组无差异。此外,MC 还改善了腱细胞的活性、胶原蛋白的生成和新生血管的形成。生物信息学相互作用表明,MC 的蛋白酶可触发信号,在血管生成、减轻炎症、促进腱母细胞活化和胶原重塑方面发挥作用。MC提取物能改善受伤肌腱的愈合,并提供令人满意的肌腱修复效果。建议进一步研究 MC 的愈合能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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