Synthesis of fluorescently labeled pyrazole derivative induceing a triple response in Arabidopsis seedlings.

IF 1.5 4区 农林科学 Q2 ENTOMOLOGY
Keimei Oh, Kai Jiang
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引用次数: 0

Abstract

A fluorescent labeled pyrazole derivative with a dansyl moiety (EH-DF) was synthesized. Design of EH-DF was carried out by using a dansyl moiety to substitute the naphthalene moiety of the parent compound (EH-1). At a concentration of 30 µM, EH-DF displayed biological activity on inducing a triple response in Arabidopsis seedlings. Compared with the non-chemical treated control, the hypocotyl length of EH-DF-treated Arabidopsis seedlings was reduced from approximately 9.2±0.7 mm to 2.4±0.2 mm. The length of the roots was reduced from 1.7±0.1 mm to 1.0±0.1 mm, and the curvature of the hook of Arabidopsis seedlings increased from 60±16 degrees to 245±35 degrees. The maxim excitation wavelength and emission wavelength of EH-DF were 350 and 535 nm, respectively. Data obtained via fluorescent microscope analysis indicated that intensive fluorescent signals of EH-DF were observed in the shoot of Arabidopsis seedlings.

Abstract Image

Abstract Image

Abstract Image

荧光标记吡唑衍生物在拟南芥幼苗中诱导三重反应的合成。
合成了一种荧光标记的丹酰吡唑衍生物。EH-DF的设计是用丹酰部分代替母体化合物EH-1的萘部分。在浓度为30µM时,EH-DF在拟南芥幼苗中表现出诱导三重反应的生物活性。与未经化学处理的对照相比,eh - df处理的拟南芥幼苗下胚轴长度从约9.2±0.7 mm缩短至2.4±0.2 mm。根长从1.7±0.1 mm减少到1.0±0.1 mm,拟南芥幼苗钩曲率从60±16度增加到245±35度。EH-DF的最大激发波长为350 nm,最大发射波长为535 nm。荧光显微镜分析数据表明,EH-DF在拟南芥幼苗的茎部中观察到强烈的荧光信号。
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来源期刊
Journal of Pesticide Science
Journal of Pesticide Science 农林科学-昆虫学
CiteScore
4.30
自引率
4.20%
发文量
28
审稿时长
18-36 weeks
期刊介绍: The Journal of Pesticide Science publishes the results of original research regarding the chemistry and biochemistry of pesticides including bio-based materials. It also covers their metabolism, toxicology, environmental fate and formulation.
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