Synthesis of fluorescently labeled pyrazole derivative induceing a triple response in Arabidopsis seedlings.

IF 1.5 4区 农林科学 Q2 ENTOMOLOGY
Keimei Oh, Kai Jiang
{"title":"Synthesis of fluorescently labeled pyrazole derivative induceing a triple response in <i>Arabidopsis</i> seedlings.","authors":"Keimei Oh,&nbsp;Kai Jiang","doi":"10.1584/jpestics.D22-006","DOIUrl":null,"url":null,"abstract":"<p><p>A fluorescent labeled pyrazole derivative with a dansyl moiety (<b>EH-DF</b>) was synthesized. Design of <b>EH-DF</b> was carried out by using a dansyl moiety to substitute the naphthalene moiety of the parent compound (<b>EH-1</b>). At a concentration of 30 µM, <b>EH-DF</b> displayed biological activity on inducing a triple response in <i>Arabidopsis</i> seedlings. Compared with the non-chemical treated control, the hypocotyl length of <b>EH-DF</b>-treated <i>Arabidopsis</i> seedlings was reduced from approximately 9.2±0.7 mm to 2.4±0.2 mm. The length of the roots was reduced from 1.7±0.1 mm to 1.0±0.1 mm, and the curvature of the hook of <i>Arabidopsis</i> seedlings increased from 60±16 degrees to 245±35 degrees. The maxim excitation wavelength and emission wavelength of <b>EH-DF</b> were 350 and 535 nm, respectively. Data obtained <i>via</i> fluorescent microscope analysis indicated that intensive fluorescent signals of <b>EH-DF</b> were observed in the shoot of <i>Arabidopsis</i> seedlings.</p>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2022-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e7/cf/jps-47-4-D22-006.PMC9716048.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pesticide Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1584/jpestics.D22-006","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

A fluorescent labeled pyrazole derivative with a dansyl moiety (EH-DF) was synthesized. Design of EH-DF was carried out by using a dansyl moiety to substitute the naphthalene moiety of the parent compound (EH-1). At a concentration of 30 µM, EH-DF displayed biological activity on inducing a triple response in Arabidopsis seedlings. Compared with the non-chemical treated control, the hypocotyl length of EH-DF-treated Arabidopsis seedlings was reduced from approximately 9.2±0.7 mm to 2.4±0.2 mm. The length of the roots was reduced from 1.7±0.1 mm to 1.0±0.1 mm, and the curvature of the hook of Arabidopsis seedlings increased from 60±16 degrees to 245±35 degrees. The maxim excitation wavelength and emission wavelength of EH-DF were 350 and 535 nm, respectively. Data obtained via fluorescent microscope analysis indicated that intensive fluorescent signals of EH-DF were observed in the shoot of Arabidopsis seedlings.

Abstract Image

Abstract Image

Abstract Image

荧光标记吡唑衍生物在拟南芥幼苗中诱导三重反应的合成。
合成了一种荧光标记的丹酰吡唑衍生物。EH-DF的设计是用丹酰部分代替母体化合物EH-1的萘部分。在浓度为30µM时,EH-DF在拟南芥幼苗中表现出诱导三重反应的生物活性。与未经化学处理的对照相比,eh - df处理的拟南芥幼苗下胚轴长度从约9.2±0.7 mm缩短至2.4±0.2 mm。根长从1.7±0.1 mm减少到1.0±0.1 mm,拟南芥幼苗钩曲率从60±16度增加到245±35度。EH-DF的最大激发波长为350 nm,最大发射波长为535 nm。荧光显微镜分析数据表明,EH-DF在拟南芥幼苗的茎部中观察到强烈的荧光信号。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Pesticide Science
Journal of Pesticide Science 农林科学-昆虫学
CiteScore
4.30
自引率
4.20%
发文量
28
审稿时长
18-36 weeks
期刊介绍: The Journal of Pesticide Science publishes the results of original research regarding the chemistry and biochemistry of pesticides including bio-based materials. It also covers their metabolism, toxicology, environmental fate and formulation.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信