A water-soluble polysaccharide from Eucommia folium: the structural characterization and anti-tumor activity in vivo.

IF 2.7 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Glycoconjugate Journal Pub Date : 2022-12-01 DOI:10.1007/s10719-022-10086-4

Zhi-Qian Yan, Su-Yun Ding, Pei Chen, Hui-Ping Liu, Meng-Li Chang, Shu-Yuan Shi

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引用次数: 3

Abstract

In this study, a water-soluble polysaccharide from Eucommia folium was extracted by hot water and purified using Sephadex G-200 gel columns. The results showed that the purified fraction (EFP) has a molecular weight of 9.98 × 10⁵ Da and consisted of rhamnose, arabinose, galactose, glucose, mannose, xylose, galacturonic acid, and glucuronic acid (molar ratio: 0.226: 1.739: 2.183: 1: 0.155: 0.321: 0.358: 0.047). The combination of infrared spectroscopy and NMR analysis proved that EFP is an acidic polysaccharide whose main chain consists of α-L-Araf-(1 → , → 3,5)-α-Araf-(1 → , → 3)-β-Galp-(1 → , → 3,6)-β-Glcp-(1 → , → 2)-α-D-Manp-(1 → , → 4)-α-GalpA-(1 → , → 2,4)-α-Rhap-(1 → . In addition, the in vivo antitumoral activity of EFP was studied using a H22 tumor-bearing mice model. EFP effectively inhibited tumor growth in mice following intragastric administration. By Combining with the results of the apoptosis assay and JC-1 staining analysis, we confirmed that EFP induces apoptosis through the mitochondrial pathway. Furthermore, cell cycle analysis demonstrated that EFP blocks the cell cycle at S phase.

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杜仲叶水溶性多糖的结构表征及体内抗肿瘤活性。

本研究采用热水提取杜仲叶水溶性多糖，并用Sephadex G-200凝胶柱进行纯化。结果表明，纯化后的EFP分子量为9.98 × 105 Da，由鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖、木糖、半乳糖醛酸和葡萄糖醛酸组成，摩尔比为0.226:1.739:2.183:1:0.155:0.321:0.358:0.047。相结合的红外光谱和核磁共振分析证明了EFP是一种酸性多糖的主链由α-L-Araf -(1→3→5)-α-Araf -(1→→3)βgalp -(1→3→6)-β-Glcp -(1→→2)α-D-Manp -(1→→4)α-GalpA -(1→→2、4)-α世行-(1→。此外，采用H22荷瘤小鼠模型研究了EFP的体内抗肿瘤活性。EFP可有效抑制小鼠灌胃后的肿瘤生长。结合凋亡实验和JC-1染色分析结果，我们证实EFP通过线粒体途径诱导细胞凋亡。此外，细胞周期分析表明EFP在S期阻断细胞周期。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Glycoconjugate Journal 生物-生化与分子生物学

CiteScore

6.00

自引率

3.30%

发文量

审稿时长

1 months

期刊介绍： Glycoconjugate Journal publishes articles and reviews on all areas concerned with: function, composition, structure, biosynthesis, degradation, interactions, recognition and chemo-enzymatic synthesis of glycoconjugates (glycoproteins, glycolipids, oligosaccharides, polysaccharides and proteoglycans), biochemistry, molecular biology, biotechnology, immunology and cell biology of glycoconjugates, aspects related to disease processes (immunological, inflammatory, arthritic infections, metabolic disorders, malignancy, neurological disorders), structural and functional glycomics, glycoimmunology, glycovaccines, organic synthesis of glycoconjugates and the development of methodologies if biologically relevant, glycosylation changes in disease if focused on either the discovery of a novel disease marker or the improved understanding of some basic pathological mechanism, articles on the effects of toxicological agents (alcohol, tobacco, narcotics, environmental agents) on glycosylation, and the use of glycotherapeutics. Glycoconjugate Journal is the official journal of the International Glycoconjugate Organization, which is responsible for organizing the biennial International Symposia on Glycoconjugates.