Distribution of emm, superantigen and other virulence genotypes and detection of phylogenetic relationships in group A streptococcal isolates.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-12-06 DOI:10.1556/030.2022.01740

Suna Kızılyıldırım, Fatih Köksal, Cansu Önlen Güneri

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引用次数: 1

Abstract

Group A streptococci are important pathogens with various virulence factors, such as M protein, superantigens, hemolysins, deoxyribonuclease, and proteases. The aims of this study are to investigate the detection of emm genotypes and other virulence genes, such as SAgs, DNase, protease, antibiotic resistance, and phylogenetic relationships in GAS strains isolated from clinical samples.Test strains were obtained from Çukurova University Balcalı Hospital and regional hospitals in Adana province. The M proteins were detected by sequence analysis of emm genes. SAgs and other virulence gene profiles were determined using the Multiplex-PCR method. The antibiotic susceptibility of the isolates was performed by the disc diffusion method and evaluated according to CLSI criteria. The PFGE method was used to determine the clonal relationship between the strains.The emm gene was positive in 86 isolates. The most common emm genotypes were emm28 (22%), emm1 (18.6%), emm12 (13.9%), and emm3 (11.6%). Also, the most common virulence genes were speG (58.1%), speC (56.9%), sdaB (53.4%), and mac (53.4%). The rates of resistance to erythromycin, clindamycin, levofloxacin, ciprofloxacin and telithromycin were 19.8%, 16.3%, 4.7%, 3.5%, and 3.5%, respectively.As a result, additional regional studies on the detection and prevalence of GAS virulence factors in Turkey are required. We believe that this study will provide valuable information for epidemiological studies on emm sequences, Sags, and other virulence factors of Streptococcus pyogenes in Turkey.

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A群链球菌分离株emm、超抗原等毒力基因型分布及系统发育关系检测

A群链球菌是具有多种毒力因子的重要病原体，如M蛋白、超抗原、溶血素、脱氧核糖核酸酶、蛋白酶等。本研究的目的是研究从临床样本分离的GAS菌株中emm基因型和其他毒力基因(如sag、DNase、蛋白酶、抗生素耐药性)的检测以及系统发育关系。试验菌株来自Çukurova巴尔卡尔伊大学医院和阿达纳省的地区医院。通过emm基因序列分析检测M蛋白。采用多重pcr法测定sag和其他毒力基因谱。采用圆盘扩散法对分离菌株进行药敏试验，并按CLSI标准进行评价。采用PFGE法确定菌株间的克隆关系。86株菌株emm基因阳性。最常见的emm基因型为emm28(22%)、emm1(18.6%)、emm12(13.9%)和emm3(11.6%)。最常见的毒力基因为speG(58.1%)、speC(56.9%)、sdaB(53.4%)和mac(53.4%)。对红霉素、克林霉素、左氧氟沙星、环丙沙星和特利霉素的耐药率分别为19.8%、16.3%、4.7%、3.5%和3.5%。因此，需要对土耳其气体毒力因素的检测和流行情况进行更多的区域研究。我们相信本研究将为土耳其化脓性链球菌的emm序列、sag等毒力因子的流行病学研究提供有价值的信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.