Development of an immunochromatographic strip test for rapid detection of a potent neutralizing anti-interferon gamma antibody in adult-onset immunodeficiency.

IF 2.1 4区医学 Q3 ALLERGY

Asian Pacific journal of allergy and immunology Pub Date : 2026-03-01 DOI:10.12932/AP-010523-1597

Umpa Yasamut, Weeraya Thongkum, Ekkarat Wongsawat, Chatchai Tayapiwatana

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引用次数: 0

Abstract

Background: Adult-onset immunodeficiency (AOID) is associated with the presence of anti-interferon gamma autoantibodies (anti-IFN-γ autoAbs), which neutralizes IFN-γ signaling and leads to susceptibility to intracellular opportunistic infections. However, measuring neutralizing autoAbs is not practical for general clinical laboratories.

Objective: This study aims to develop a competitive immunochromatographic (IC) strip test for detecting autoAbs that recognize the same epitope as a potent neutralizing antibody.

Methods: The competitive IC strip test detecting autoAbs that recognize the same epitope as mouse anti-IFN-γ monoclonal antibody, clone B27 (B27 mAb) was fabricated and used to determine B27 epitope recognizing autoAb (B27 AAb) in AOID plasma. The competitive ELISA was used as a comparative method. AR patients.

Results: The efficacy of the IC strip test was compared with competitive ELISA and found a percent positive agreement of 91.30%, a percent negative agreement of 79.31%, and a percent overall agreement of 84.62%.

Conclusions: The results from the competitive IC strip test were consistent with those from competitive ELISA, indicating that the generated IC strip could detect B27 AAb in AOID plasma.

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一种用于快速检测成人免疫缺陷中有效中和抗干扰素γ抗体的免疫层析试纸。

背景：成人性免疫缺陷（AOID）与抗干扰素γ自身抗体（抗IFN-γ自身抗体）的存在有关，其中和IFN-γ信号并导致对细胞内机会性感染的易感性。然而，测量中和自身抗体是不实际的一般临床实验室。目的：本研究旨在建立一种竞争性免疫层析（IC）试纸，用于检测识别与强效中和抗体相同表位的自身抗体。方法：制备识别与小鼠抗ifn -γ单克隆抗体相同表位的自身抗体的竞争性IC条试验，克隆B27 (B27 mAb)，用于检测小鼠血浆中识别B27表位的自身抗体（B27 AAb）。采用竞争性ELISA法进行比较。基于“增大化现实”技术的患者。结果：与竞争性ELISA法比较，IC条试验的阳性率为91.30%，阴性率为79.31%，总体符合率为84.62%。结论：竞争性IC试纸与竞争性ELISA检测结果一致，表明所制备的IC试纸能检测出血浆中B27 AAb。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Asian Pacific journal of allergy and immunology 医学-过敏

CiteScore

12.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Asian Pacific Journal of Allergy and Immunology (APJAI) is an online open access journal with the recent impact factor (2018) 1.747 APJAI published 4 times per annum (March, June, September, December). Four issues constitute one volume. APJAI publishes original research articles of basic science, clinical science and reviews on various aspects of allergy and immunology. This journal is an official journal of and published by the Allergy, Asthma and Immunology Association, Thailand. The scopes include mechanism, pathogenesis, host-pathogen interaction, host-environment interaction, allergic diseases, immune-mediated diseases, epidemiology, diagnosis, treatment and prevention, immunotherapy, and vaccine. All papers are published in English and are refereed to international standards.