The possible protective role of pimpinella anisum oil versus selenium on aspartame induced changes in rat cerebellar cortex: histological, immunohistochemical and electron microscopic study.

IF 1.1 4区 医学 Q4 MICROSCOPY
Amira I Shrief, Ahmed A M Abdel-Hamid, Am Moustafa, E El-Mohandes
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引用次数: 0

Abstract

Aspartame (ASP) is an artificial sweeter. Chronic use of ASP has a harmful effect on cerebellar cortex. Anisum oil and selenium (SE) are antioxidant substances. Therefore, the present study was performed to study the possible protective role of anisum oil versus selenium on aspartame-induced changes in rat cerebellar cortex. Rats were divided into four main groups. Group I (Control group). Group II received 250 mg/kg/day aspartame once daily for 2 months. Group III received 0.5 ml/kg/day anisum 2 h before aspartame administration. Group IV received 0.5 mg/kg/day selenium 2 h before aspartame administration. The administration of Asp for 2 months (group II) resulted in cerebellar histopathological changes in the form of deformed Purkinje and granule cells. Ultrastructurally, Purkinje cells had irregular nuclei, dilated cisternae of rough endoplasmic reticulum, dilated saccules of Golgi apparatus, mitochondria with destroyed cristae. In addition, granule cells appeared shrunken with irregular nuclei. Aspartame and anisum oil treated group (group III) showed partial improvement. Examination of ASP and SE treated group (group IV) showed that cerebellar cortex was nearly similar to control. In conclusion, Anisum oil and selenium could protect against ASP-induced cerebellar damage. The protective effect of selenium is better than anisum oil.

茴香油与硒对阿斯巴甜诱导的大鼠小脑皮层变化的保护作用:组织学、免疫组化和电镜研究。
阿斯巴甜(ASP)是一种人工甜味剂。长期使用ASP对小脑皮质有不良影响。茴香油和硒(SE)是抗氧化物质。因此,本研究旨在探讨茴香油与硒对阿斯巴甜诱导的大鼠小脑皮层变化的保护作用。大鼠被分为四组。第一组(对照组)。II组给予阿斯巴甜250 mg/kg/天,每日1次,连续2个月。第三组在阿斯巴甜给药前2 h给予茴香0.5 ml/kg/天。IV组在阿斯巴甜给药前2 h给予0.5 mg/kg/天硒。给药2个月(II组)后,小鼠小脑浦肯野细胞和颗粒细胞发生变形。超微结构上浦肯野细胞细胞核不规则,粗内质网池扩张,高尔基体小囊扩张,线粒体嵴破坏。颗粒细胞萎缩,细胞核不规则。阿斯巴甜和茴香油处理组(III组)有部分改善。ASP和SE治疗组(IV组)检查显示,小脑皮质与对照组基本相似。综上所述,茴香油和硒对asp诱导的小脑损伤具有保护作用。硒的保护作用优于茴香油。
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来源期刊
Ultrastructural Pathology
Ultrastructural Pathology 医学-病理学
CiteScore
2.00
自引率
10.00%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.
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