Inactivation of a wheat protein kinase gene confers broad-spectrum resistance to rust fungi.

IF 45.5 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cell Pub Date : 2022-08-04 DOI:10.1016/j.cell.2022.06.027

Ning Wang, Chunlei Tang, Xin Fan, Mengying He, Pengfei Gan, Shan Zhang, Zeyu Hu, Xiaodong Wang, Tong Yan, Weixue Shu, Ligang Yu, Jinren Zhao, Jiani He, Lili Li, Jianfeng Wang, Xueling Huang, Lili Huang, Jian-Min Zhou, Zhensheng Kang, Xiaojie Wang

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引用次数: 49

Abstract

Wheat crops are frequently devastated by pandemic stripe rust caused by Puccinia striiformis f. sp. tritici (Pst). Here, we identify and characterize a wheat receptor-like cytoplasmic kinase gene, TaPsIPK1, that confers susceptibility to this pathogen. PsSpg1, a secreted fungal effector vital for Pst virulence, can bind TaPsIPK1, enhance its kinase activity, and promote its nuclear localization, where it phosphorylates the transcription factor TaCBF1d for gene regulation. The phosphorylation of TaCBF1d switches its transcriptional activity on the downstream genes. CRISPR-Cas9 inactivation of TaPsIPK1 in wheat confers broad-spectrum resistance against Pst without impacting important agronomic traits in two years of field tests. The disruption of TaPsIPK1 leads to immune priming without constitutive activation of defense responses. Taken together, TaPsIPK1 is a susceptibility gene known to be targeted by rust effectors, and it has great potential for developing durable resistance against rust by genetic modifications.

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小麦蛋白激酶基因的失活赋予了对锈菌的广谱抗性。

小麦作物经常遭受由小麦条锈病(Pst)引起的大流行条锈病的破坏。在这里，我们鉴定并表征了小麦受体样细胞质激酶基因TaPsIPK1，该基因对这种病原体具有易感性。PsSpg1是一种对Pst毒力至关重要的分泌真菌效应物，可以结合TaPsIPK1，增强其激酶活性，促进其核定位，在核定位中磷酸化转录因子TaCBF1d进行基因调控。TaCBF1d的磷酸化将其转录活性转向下游基因。在两年的田间试验中，CRISPR-Cas9灭活小麦TaPsIPK1，在不影响重要农艺性状的情况下，获得了对Pst的广谱抗性。TaPsIPK1的破坏导致免疫启动而不构成防御反应的激活。综上所述，TaPsIPK1是一个已知的锈病效应物的易感基因，它有很大的潜力通过遗传修饰发展出持久的抗锈病能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell 生物-生化与分子生物学

CiteScore

110.00

自引率

0.80%

发文量

396

审稿时长

2 months

期刊介绍： Cells is an international, peer-reviewed, open access journal that focuses on cell biology, molecular biology, and biophysics. It is affiliated with several societies, including the Spanish Society for Biochemistry and Molecular Biology (SEBBM), Nordic Autophagy Society (NAS), Spanish Society of Hematology and Hemotherapy (SEHH), and Society for Regenerative Medicine (Russian Federation) (RPO). The journal publishes research findings of significant importance in various areas of experimental biology, such as cell biology, molecular biology, neuroscience, immunology, virology, microbiology, cancer, human genetics, systems biology, signaling, and disease mechanisms and therapeutics. The primary criterion for considering papers is whether the results contribute to significant conceptual advances or raise thought-provoking questions and hypotheses related to interesting and important biological inquiries. In addition to primary research articles presented in four formats, Cells also features review and opinion articles in its "leading edge" section, discussing recent research advancements and topics of interest to its wide readership.