Role of aquaporin 5 and glandular blood flow in the acetylcholine-induced secretion of saliva in rats.

IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Mst Tahmina Akter, Akihiro Nezu, Tetsuya Akamatsu, Akihiko Tanimura
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引用次数: 0

Abstract

To clarify the role of the aquaporin 5 (AQP5) in salivary secretion, we evaluated acetylcholine (ACh)-induced secretion in Sprague-Dawley (SD) rats, rats expressing a low level of AQP5 protein (AQP5/low SD) which developed from SD rats, and Wistar/ST rats. The salivary secretion in AQP5/low SD rats in response to infusions of low-dose ACh (60-120 nmol/min) was 27-42% of that in SD rats. By contrast, Wistar/ST rats exhibited comparable secretion to that of SD rats in response to low-doses ACh, despite their low-level expression of AQP5. Experiments using spectrofluorometry and RT-PCR demonstrated no differences in the ACh-induced Ca2+ responses or the mRNA expression of muscarinic receptor, Cl- channel, or cotransporter between these strains. These findings imply that factors other than the function of salivary acinar cells regulates the secretion in response to weak stimuli. Monitoring of the hemodynamics in the submandibular gland revealed that low-doses ACh induced different patterns of the fluctuations in the blood flow in these strains. The blood flow decreased below the resting level in AQP5/low SD rats, but remained mostly above the resting level in Wistar/ST rats. The present study reveals that the contribution of AQP5-dependent transport of water is altered by stimulus intensity and blood flow.

水通道蛋白5和腺体血流在大鼠乙酰胆碱诱导的唾液分泌中的作用。
为了阐明水通道蛋白5 (AQP5)在唾液分泌中的作用,我们对Sprague-Dawley (SD)大鼠、低水平表达AQP5蛋白(AQP5/低SD)大鼠和Wistar/ST大鼠的乙酰胆碱(ACh)诱导分泌进行了研究。低剂量乙酰胆碱(60 ~ 120 nmol/min)灌胃后,AQP5/低SD大鼠的唾液分泌量为SD大鼠的27 ~ 42%。相比之下,Wistar/ST大鼠对低剂量乙酰胆碱的分泌与SD大鼠相当,尽管它们的AQP5表达水平较低。荧光光谱法和RT-PCR实验显示,这些菌株之间ach诱导的Ca2+反应或毒蕈碱受体、Cl-通道或共转运体的mRNA表达没有差异。这些发现表明,除了唾液腺泡细胞的功能外,还有其他因素调节了对弱刺激的分泌。对颌下腺血流动力学的监测显示,低剂量乙酰胆碱诱导这些菌株血流波动的不同模式。AQP5/低SD大鼠的血流量低于静息水平,而Wistar/ST大鼠的血流量大多高于静息水平。本研究表明,刺激强度和血流改变了aqp5依赖性水转运的贡献。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomedical Research-tokyo
Biomedical Research-tokyo 医学-医学:研究与实验
CiteScore
2.40
自引率
0.00%
发文量
19
审稿时长
>12 weeks
期刊介绍: Biomedical Research is peer-reviewed International Research Journal . It was first launched in 1990 as a biannual English Journal and later became triannual. From 2008 it is published in Jan-Apr/ May-Aug/ Sep-Dec..
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