State-of-the-art liver disease research using liver-on-a-chip.

IF 5 3区 医学 Q2 IMMUNOLOGY
Sayaka Deguchi, Kazuo Takayama
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引用次数: 0

Abstract

To understand disease pathophysiologies, models that recapitulate human functions are necessary. In vitro models that consist of human cells are preferred to ones using animal cells, because organ functions can vary from species to species. However, conventional in vitro models do not recapitulate human organ functions well. Organ-on-a-chip technology provides a reliable in vitro model of the functional units of human organs. Organ-on-a-chip technology uses microfluidic devices and their accessories to impart organ functions to human cells. Using microfluidic devices, we can co-culture multiple cell types that compose human organs. Moreover, we can culture human cells under physiologically relevant stresses, such as mechanical and shear stresses. Current organ-on-a-chip technology can reproduce the functions of several organs including the liver. Because it is difficult to maintain the function of human hepatocytes, which are the gold standard of in vitro liver models, under conventional culture conditions, the application of liver-on-a-chips to liver disease research is expected. This review introduces the current status and future prospects of liver-on-a-chips in liver disease research.

Abstract Image

Abstract Image

Abstract Image

利用片上肝脏进行最先进的肝病研究。
要了解疾病的病理生理学,就必须建立能再现人体功能的模型。由人体细胞组成的体外模型比使用动物细胞的模型更受欢迎,因为器官功能会因物种而异。然而,传统的体外模型并不能很好地再现人体器官功能。芯片上器官技术提供了可靠的人体器官功能单元体外模型。芯片上器官技术利用微流体设备及其附件将器官功能赋予人体细胞。利用微流体设备,我们可以共同培养组成人体器官的多种细胞类型。此外,我们还可以在生理应力(如机械应力和剪切应力)条件下培养人体细胞。目前的芯片上器官技术可以再现包括肝脏在内的多个器官的功能。人肝细胞是体外肝脏模型的金标准,在传统培养条件下很难维持其功能,因此肝脏芯片有望应用于肝脏疾病研究。本综述介绍了片上肝脏在肝病研究中的应用现状和前景。
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来源期刊
CiteScore
11.10
自引率
1.20%
发文量
45
审稿时长
11 weeks
期刊介绍: Inflammation and Regeneration is the official journal of the Japanese Society of Inflammation and Regeneration (JSIR). This journal provides an open access forum which covers a wide range of scientific topics in the basic and clinical researches on inflammation and regenerative medicine. It also covers investigations of infectious diseases, including COVID-19 and other emerging infectious diseases, which involve the inflammatory responses. Inflammation and Regeneration publishes papers in the following categories: research article, note, rapid communication, case report, review and clinical drug evaluation.
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