Development, systematic optimisation and biofilm disruption activity of eugenol-based nanosized emulsions stabilised with Tween 80.

IF 3 4区 医学 Q2 CHEMISTRY, APPLIED
Journal of microencapsulation Pub Date : 2023-11-01 Epub Date: 2023-08-11 DOI:10.1080/02652048.2023.2244094
Chandra Lekha Putta, Syed Nazrin Ruhina Rahman, Payel Chakraborty, Tamilvanan Shunmugaperumal
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引用次数: 0

Abstract

The aims of this study were to systematically optimise a formula for eugenol emulsions via face-centered central composite design and to assess the activity against two-different bacterial strains (Staphylococcus aureus and Propionibacterium acnes) present at planktonic and biofilm forms. The molecular interaction of excipients, mean particle size (MPS) including zeta potential (ZP), drug entrapment efficiency (DEE) and in vitro drug release of optimised emulsions was done using FT-IR, Malvern Zetasizer, ultracentrifugation technique and membrane-free dissolution model, respectively. The emulsions consisted of 151.3 ± 1.45 nm MPS, -21.3 ± 1.25 mV ZP and 93.98 ± 1.41% DEE values. On storage of emulsions at 25 °C for 3 months, the value of DEE was found to be 72.12 ± 2.82%. The Tween 80 emulsifier film coverage onto the dispersed eugenol droplets of emulsions delayed significantly the drug release (12%-19%) compared to the drug release occurred from pure eugenol. The treatment of planktonic S. aureus and P. acnes with diluted eugenol emulsions showed the minimum inhibitory concentration and minimum bactericidal concentration values at 1.25-2.5 mg/ml whereas it occurred at 10 mg/ml for pure eugenol. Treating the biofilms with eugenol emulsions (1-2 mg/ml) yielded 59-70% minimum biofilm eradication concentration but 10 mg/ml pure eugenol showed 60%. Hence, the eugenol emulsions displayed antibacterial activity and could be projected as an antibiofilm or biofilm disruption agent.

吐温80稳定丁香酚基纳米乳液的开发、系统优化和生物膜破坏活性。
本研究的目的是通过面心中心复合材料设计系统地优化丁香酚乳液的配方,并评估其对浮游和生物膜形式的两种不同菌株(金黄色葡萄球菌和痤疮丙酸杆菌)的活性。分别使用FT-IR、Malvern-Zetasizer、超速离心技术和无膜溶出模型研究了辅料、平均粒径(MPS)(包括ζ电位(ZP))、药物包封率(DEE)和优化乳液的体外药物释放的分子相互作用。乳液由151.3 ± 1.45 nm MPS,-21.3 ± 1.25 mV ZP和93.98 ± 1.41%DEE值。乳液储存温度为25 3°C 月,DEE的值为72.12 ± 与纯丁香酚的药物释放相比,Tween 80乳化剂膜覆盖在乳液的分散丁香酚液滴上显著延迟了药物释放(12%-19%)。稀释丁香酚乳剂对浮游金黄色葡萄球菌和痤疮葡萄球菌的处理显示出最小抑制浓度和最小杀菌浓度值为1.25-2.5 mg/ml,而在10 纯丁香酚为mg/ml。用丁香酚乳液处理生物膜(1-2 mg/ml)产生59-70%的最小生物膜根除浓度,但是10 mg/ml纯丁香酚显示60%。因此,丁香酚乳液显示出抗菌活性,可以作为抗菌膜或生物膜破坏剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of microencapsulation
Journal of microencapsulation 工程技术-工程:化工
CiteScore
6.30
自引率
2.60%
发文量
39
审稿时长
3 months
期刊介绍: The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.
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