Evolution of YacG to safeguard DNA gyrase from external perturbation

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Research in microbiology Pub Date : 2023-09-01 DOI:10.1016/j.resmic.2023.104093

Priti Biswas , Sugopa Sengupta , Valakunja Nagaraja

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引用次数: 0

Abstract

Cells have evolved strategies to safeguard their genome integrity. We describe a mechanism to counter double strand breaks in the chromosome that involves the protection of an essential housekeeping enzyme from external agents. YacG is a DNA gyrase inhibitory protein from Escherichia coli that protects the bacterium from the cytotoxic effects of catalytic inhibitors as well as cleavage-complex stabilizers of DNA gyrase. By virtue of blocking the primary DNA binding site of the enzyme, YacG prevents the accumulation of double strand breaks induced by gyrase poisons. It also enables the bacterium to resist the growth-inhibitory property of novobiocin. Gyrase poison-induced oxidative stress upregulates YacG production, probably as a cellular response to counter DNA damage. YacG-mediated protection of the genome is specific for gyrase targeting agents as the protection is not observed from the action of general DNA damaging agents. YacG also intensifies the transcription stress induced by rifampicin substantiating the importance of gyrase activity during transcription. Although essential for bacterial survival, DNA gyrase often gets entrapped by external inhibitors and poisons, resulting in cell death. The existence of YacG to specifically protect an essential housekeeping enzyme might be a strategy adopted by bacteria for competitive fitness advantage.

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YacG的进化以保护DNA旋转酶免受外部干扰。

细胞已经进化出了保护其基因组完整性的策略。我们描述了一种对抗染色体双链断裂的机制，该机制涉及保护一种重要的家政酶免受外部因素的影响。YacG是一种来自大肠杆菌的DNA回旋酶抑制蛋白，可保护细菌免受催化抑制剂和DNA回旋酶切割复合物稳定剂的细胞毒性作用。通过阻断酶的初级DNA结合位点，YacG可以防止由旋转酶毒素诱导的双链断裂的积累。它还使细菌能够抵抗新生物素的生长抑制特性。Gyrase毒素诱导的氧化应激上调YacG的产生，可能是对抗DNA损伤的细胞反应。YacG介导的基因组保护对gyrase靶向剂是特异性的，因为从一般DNA损伤剂的作用中没有观察到这种保护。YacG还增强了利福平诱导的转录应激，证实了旋转酶活性在转录过程中的重要性。尽管DNA聚合酶对细菌的生存至关重要，但它经常被外部抑制剂和毒素包裹，导致细胞死亡。YacG的存在可以特异性地保护一种重要的内务酶，这可能是细菌为了竞争适应度优势而采取的一种策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Research in microbiology 生物-微生物学

CiteScore

4.10

自引率

3.80%

发文量

审稿时长

16 days

期刊介绍： Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.