RT-qPCR Analysis of LAMP3 (CD208) Gene Expression in Oral Lichen Planus and Oral Squamous Cell Carcinoma.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Farnaz Mohajertehran, Nooshin Mohtasham, Mojtaba Ahmadi, Mehdi Shahabinejad, Maryam Mohammadi
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引用次数: 0

Abstract

Background: Many new studies have been conducted on cellular proteins to use them as prognostic markers or in target therapy through determining the increase or decrease in their expression in the lichen planus and OSCC. LAMP3 protein is one of these proteins which has been recently considered. Thus, considering the unknown etiology of lichen planus, significance of their early diagnosis and treatment and lack of a suitable and final treatment for this disease and oral cancers, and preventing the progression of lichen planus, which can turn into OSCC, we decided to investigate the level of expression of this gene and its effect on the progression, study the connection between these two conditions and the probable factors contributing to their etiopathogenesis.

Methods: In this study, ninety-four paraffin blocks tissue samples of patients were obtained together with their demographic documents. LAMP3 expression was measured RT-qPCR method.

Results: The results show that there is not any significant difference between age and sex population of our study. in squamous cell carcinoma the amount of expression of LAMP3 was higher than lichen planus and healthy margin. Average LAMP3 Gene expression in grade III was higher than group grade I & II in which considering significant level of 5%, it is statistically significant.

Conclusions: According to the findings of this study, it can be concluded that the expression of the LAMP3 gene in SCC lesions is higher than in healthy tissue. Hence, LAMP3 gene expression can be used as a diagnostic biomarker.

口腔扁平苔藓和口腔鳞状细胞癌中LAMP3(CD208)基因表达的RT-qPCR分析。
背景:已经对细胞蛋白进行了许多新的研究,通过确定其在扁平苔藓和OSCC中表达的增加或减少,将其用作预后标志物或靶向治疗。LAMP3蛋白是最近被考虑的这些蛋白之一。因此,考虑到扁平苔藓的病因未知,其早期诊断和治疗的重要性,以及对该疾病和口腔癌缺乏合适的最终治疗方法,以及预防扁平苔藓的进展(可转变为OSCC),我们决定研究该基因的表达水平及其对进展的影响,研究这两种情况之间的联系以及可能导致其发病的因素。方法:在本研究中,获得了94份患者的石蜡块组织样本及其人口统计学文件。采用RT-qPCR方法测定LAMP3的表达。结果:研究结果表明,本研究的年龄和性别人群之间没有任何显著差异。在鳞状细胞癌中,LAMP3的表达量高于扁平苔藓和健康边缘。LAMP3基因在III级的平均表达高于I级和II级组,考虑到5%的显著水平,具有统计学意义。结论:根据本研究的结果,可以得出结论,LAMP3基因在SCC病变中的表达高于在健康组织中的表达。因此,LAMP3基因表达可以用作诊断生物标志物。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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