Association of lncRNA MEG3 Rs7158663 Polymorphism and Serum Expression with Colorectal Cancer in Egyptian Patients.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Reports of Biochemistry and Molecular Biology Pub Date : 2023-04-01 DOI:10.52547/rbmb.12.1.102

Mona Elhelaly Elsherbeny, Alyaa Ramadan Elsergany, Olfat Gamil Shaker

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引用次数: 0

Abstract

Background: Colorectal cancer (CRC) is considered the third most common cancer around the world and second in terms of mortality. A significant aspect in its development is genetics. The risk of CRC and other clinicopathologic characteristics were investigated in this work in relation to the long non-coding RNA (lncRNA) MEG3 rs7158663 polymorphism, MEG3 expression, in an Egyptian population.

Methods: 160 CRC patients and 160 healthy controls were enrolled in this case-control study. The lncRNA MEG3 rs7158663 was examined using TaqMan Real-time PCR. RT-PCR was used to assess the levels of serum MEG3 expression.

Results: A significant higher expression of 'A' allele (risk allele) and A/A genotype in CRC cases vs. control subjects (P <0.001) Participants with A/A genotype had 4.8 times higher odds to exhibit CRC. Serum MEG3 gene expression was generally low in CRC patients, and it was considerably lower in those with the rs7158663 AA genotype than those with the GG genotype (P <0.001). It was found that CRC patients with the rs7158663 GA genotype had lower serum MEG3 expression levels than those with the GG genotype (P <0.001).

Conclusions: MEG3 low expression and MEG3 rs7158663 (AA) were associated with CRC risk in Egyptian patients and may serve as a diagnostic and prognostic marker for CRC patients.

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lncRNA-MEG3Rs7158663多态性与埃及结直肠癌癌症患者血清表达的相关性。

背景：癌症（CRC）被认为是世界上第三大最常见的癌症，死亡率排名第二。其发展的一个重要方面是遗传学。在这项工作中，研究了埃及人群中与长非编码RNA（lncRNA）MEG3 rs7158663多态性（MEG3表达）相关的CRC风险和其他临床病理特征。方法：160名CRC患者和160名健康对照者参加本病例对照研究。使用TaqMan实时PCR检测lncRNA MEG3 rs7158663。RT-PCR用于评估血清MEG3的表达水平。结果：CRC患者的“A”等位基因（风险等位基因）和A/A基因型的表达显著高于对照组（P结论：MEG3低表达和MEG3 rs7158663（AA）与埃及患者的CRC风险相关，可作为CRC患者的诊断和预后标志。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

2.80

自引率

23.50%

发文量

审稿时长

10 weeks

期刊介绍： The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.