The histone demethylase KdmB is part of a trimeric protein complex and mediates virulence and mycotoxin production in Penicillium expansum

IF 2.4 3区生物学 Q3 GENETICS & HEREDITY

Fungal Genetics and Biology Pub Date : 2023-09-16 DOI:10.1016/j.fgb.2023.103837

Dianiris Luciano-Rosario , Omer Barda , Joanna Tannous , Dean Frawley , Özgür Bayram , Dov Prusky , Edward Sionov , Nancy P. Keller

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引用次数: 1

Abstract

Epigenetic modification of chromosome structure has increasingly been associated with alterations in secondary metabolism and sporulation defects in filamentous fungal pathogens. Recently, the epigenetic reader protein SntB was shown to govern virulence, spore production and mycotoxin synthesis in the fruit pathogen Penicillium expansum. Through immunoprecipitation-coupled mass spectrometry, we found that SntB is a member of a protein complex with KdmB, a histone demethylase and the essential protein RpdA, a histone deacetylase. Deletion of kdmB phenocopied some but not all characteristics of the ΔsntB mutant. KdmB deletion strains exhibited reduced lesion development on Golden Delicious apples and this was accompanied by decreased production of patulin and citrinin in host tissue. In addition, ΔkdmB mutants were sensitive to several cell wall stressors which possibly contributed to the decreased virulence observed on apples. Slight differences in spore production and germination rates of ΔkdmB mutants in vitro did not impact overall diameter growth in culture.

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组蛋白去甲基酶KdmB是三聚蛋白复合体的一部分，介导扩展青霉的毒力和真菌毒素的产生。

染色体结构的表观遗传学修饰越来越多地与丝状真菌病原体的次生代谢和孢子形成缺陷有关。最近，表观遗传阅读蛋白SntB被证明控制果实病原体扩展青霉的毒力、孢子产生和真菌毒素合成。通过免疫沉淀-质谱联用，我们发现SntB是与组蛋白脱甲基酶KdmB和组蛋白脱乙酰酶必需蛋白RpdA组成的蛋白质复合体的一员。kdmB的缺失现象复制了ΔsntB突变体的一些但不是全部特征。KdmB缺失菌株在金苹果上表现出病变发展减少，这伴随着寄主组织中展青霉素和桔霉素的产生减少。此外，ΔkdmB突变体对几种细胞壁应激源敏感，这可能导致在苹果上观察到的毒力降低。ΔkdmB突变体在体外孢子产生和发芽率方面的微小差异不会影响培养基中的总直径生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fungal Genetics and Biology 生物-遗传学

CiteScore

6.20

自引率

3.30%

发文量

审稿时长

85 days

期刊介绍： Fungal Genetics and Biology, formerly known as Experimental Mycology, publishes experimental investigations of fungi and their traditional allies that relate structure and function to growth, reproduction, morphogenesis, and differentiation. This journal especially welcomes studies of gene organization and expression and of developmental processes at the cellular, subcellular, and molecular levels. The journal also includes suitable experimental inquiries into fungal cytology, biochemistry, physiology, genetics, and phylogeny. Fungal Genetics and Biology publishes basic research conducted by mycologists, cell biologists, biochemists, geneticists, and molecular biologists. Research Areas include: • Biochemistry • Cytology • Developmental biology • Evolutionary biology • Genetics • Molecular biology • Phylogeny • Physiology.