Prolonged alcohol consumption influences microRNA expression in the nucleus accumbens of the rat brain.

Q3 Biochemistry, Genetics and Molecular Biology
M I Airapetov, S O Eresko, S A Shamaeva, N M Matveev, E R Bychkov, A A Lebedev, P D Shabanov
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引用次数: 0

Abstract

The microRNA (miR) species analyzed in this study are involved in molecular mechanisms of TLR4 and TLR7 signaling, mediating the development of neuroinflammation and neurodegeneration. We have investigated the expression levels of miR-let7b, miR-96, miR-182, miR-155, and the mRNA content of HMGB1, TLR3, TLR4 in the nucleus accumbens (NAc) of the brain of rats exposed to long-term alcoholization. The long-term alcoholization caused a decrease in miR-let7b, miR-96, miR-182, and TLR7 mRNA levels; this was accompanied by an increase in miR-155, TLR4, and Hmgb1 mRNA levels in the NAc of rat brain. TLR7 is functionally linked to miR-let7b. The data of a simultaneous decrease in miR-let7b and TLR7 mRNA are of interest for further studies; they may indicate on the lack of functionally significant links between Hmgb1 and the miR-let7b-TLR7 system in NAc. The existing evidence of a functional relationship between TLR4 with miR-155 and miR-182 and our observations on their expression changes during chronic alcoholization are very interesting and require further investigation. The suggestion about the development of neuroinflammatory process in NAc under prolonged alcohol exposure are relevant for studying the level of TLR gene expression in NAc, as well as the expression of miR species, which may have a functional relationship with the TLR system.

长时间饮酒影响大鼠脑伏隔核中microRNA的表达。
本研究分析的microRNA (miR)物种参与了TLR4和TLR7信号转导的分子机制,介导了神经炎症和神经退行性变的发生。我们研究了长期酒精中毒大鼠大脑伏隔核(NAc)中miR-let7b、miR-96、miR-182、miR-155的表达水平以及HMGB1、TLR3、TLR4的mRNA含量。长期酒精化导致miR-let7b、miR-96、miR-182和TLR7 mRNA水平降低;这伴随着大鼠脑NAc中miR-155、TLR4和Hmgb1 mRNA水平的升高。TLR7与miR-let7b有功能关联。miR-let7b和TLR7 mRNA同时降低的数据值得进一步研究;它们可能表明在NAc中Hmgb1与miR-let7b-TLR7系统之间缺乏功能上显著的联系。TLR4与miR-155和miR-182之间存在功能关系的现有证据以及我们对慢性酒精化过程中它们的表达变化的观察是非常有趣的,需要进一步研究。关于NAc在长时间酒精暴露下神经炎症过程发生的提示,对于研究NAc中TLR基因的表达水平以及miR物种的表达可能与TLR系统有功能上的关系具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
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