In Vitro Synergistic Antibacterial and Anti-Inflammatory Effects of Nisin and Lactic Acid in Yogurt against Helicobacter pylori and Human Gastric Cells.

IF 4.2 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY
Seo Gu Han, Hyuk Cheol Kwon, Do Hyun Kim, Seong Joon Hong, Sung Gu Han
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Abstract

Helicobacter pylori is a bacterium that naturally thrives in acidic environments and has the potential to induce various gastrointestinal disorders in humans. The antibiotic therapy utilized for treating H. pylori can lead to undesired side effects, such as dysbiosis in the gut microbiota. The objective of our study was to explore the potential antibacterial effects of nisin and lactic acid (LA) in yogurt against H. pylori. Additionally, we investigated the anti-inflammatory effects of nisin and LA in human gastric (AGS) cells infected with H. pylori. Nisin and LA combination showed the strongest inhibitory activity, with confirmed synergy at 0.375 fractional inhibitory concentration index. Also, post-fermented yogurt with incorporation of nisin exhibited antibacterial effect against H. pylori. The combination of nisin and LA resulted in a significant reduction of mRNA levels of bacterial toxins of H. pylori and pro-inflammatory cytokines in AGS cells infected with H. pylori. Furthermore, this also increased bacterial membrane damage, which led to DNA and protein leakage in H. pylori. Overall, the combination of nisin and LA shows promise as an alternative therapy for H. pylori infection. Additionally, the incorporation of nisin into foods containing LA presents a potential application. Further studies, including animal research, are needed to validate these findings and explore clinical applications.

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酸奶中Nisin和乳酸对幽门螺杆菌和人胃细胞的体外协同抗菌和抗炎作用。
幽门螺杆菌是一种在酸性环境中自然繁殖的细菌,有可能引起人类各种胃肠道疾病。用于治疗幽门螺杆菌的抗生素治疗可能导致不希望的副作用,例如肠道微生物群的生态失调。本研究的目的是探讨酸奶中乳酸菌素和乳酸(LA)对幽门螺杆菌的潜在抗菌作用。此外,我们还研究了nisin和LA对幽门螺杆菌感染的人胃(AGS)细胞的抗炎作用。Nisin与LA联合抑菌活性最强,抑菌分数浓度指数为0.375。同时,乳杆菌素的加入对幽门螺杆菌也有一定的抑菌作用。nisin和LA联合使用可显著降低幽门螺杆菌感染AGS细胞中幽门螺杆菌细菌毒素mRNA水平和促炎细胞因子水平。此外,这也增加了细菌膜的损伤,导致幽门螺杆菌中DNA和蛋白质的泄漏。总的来说,nisin和LA联合应用有望成为治疗幽门螺杆菌感染的一种替代疗法。此外,将nisin掺入含有LA的食品中具有潜在的应用前景。需要进一步的研究,包括动物研究,来验证这些发现并探索临床应用。
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来源期刊
Food Science of Animal Resources
Food Science of Animal Resources Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
6.70
自引率
6.70%
发文量
75
期刊介绍: Food Science of Animal Resources (Food Sci. Anim. Resour.) is an international, peer-reviewed journal publishing original research and review articles on scientific and technological aspects of chemistry, biotechnology, processing, engineering, and microbiology of meat, egg, dairy, and edible insect/worm products.
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