Baicalin, silver titanate, Bletilla striata polysaccharide and carboxymethyl chitosan in a porous sponge dressing for burn wound healing

IF 4.2 2区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Integrative Medicine-Jim Pub Date : 2023-09-01 DOI:10.1016/j.joim.2023.07.002

Yan-rong Gong , Cheng Zhang , Xing Xiang , Zhi-bo Wang , Yu-qing Wang , Yong-hua Su , Hui-qing Zhang

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引用次数: 0

Abstract

Objective

This study tests the efficacy of Bletilla striata polysaccharide (BSP), carboxymethyl chitosan (CMC), baicalin (BA) and silver titanate (ST) in a wound dressings to fight infection, promote healing and provide superior biocompatibility.

Methods

The antibacterial activity of BA and ST was evaluated in vitro using the inhibition zone method. BA/ST/BSP/CMC porous sponge dressings were prepared and characterized. The biocompatibility of BA/ST/BSP/CMC was assessed using the cell counting kit-8 assay. The therapeutic effect of BA/ST/BSP/CMC was further investigated using the dorsal skin burn model in Sprague-Dawley rats.

Results

The wound dressing had good antibacterial activity against Escherichia coli and Staphylococcus aureus through BA and ST, while the combination of BSP and CMC played an important role in promoting wound healing. The BA/ST/BSP/CMC porous sponge dressings were prepared using a freeze-drying method with the concentrations of BA and ST at 20 and 0.83 mg/mL, respectively, and the optimal ratio of 5% BSP to 4% CMC was 1:3. The average porosity, water absorption and air permeability of BA/ST/BSP/CMC porous sponge dressings were measured to be 90.43%, 746.1% and 66.60%, respectively. After treatment for 3 and 7 days, the healing rates of the BA/ST/BSP/CMC group and BA/BSP/CMC group were significantly higher than those of the normal saline (NS) group and silver sulfadiazine (SSD) group (P < 0.05). Interleukin-1β expression in the BA/ST/BSP/CMC group at 1 and 3 days was significantly lower than that in the other three groups (P < 0.05). After being treated for 3 days, vascular endothelial growth factor expression in the BA/BSP/CMC group and BA/ST/BSP/CMC group was significantly higher than that in the NS group and SSD group (P < 0.05). Inspection of histological sections showed that the BA/ST/BSP/CMC group and BA/BSP/CMC group began to develop scabbing and peeling of damaged skin after 3 days of treatment, indicating accelerated healing relative to the NS group and SSD group.

Conclusion

The optimized concentration of BA/ST/BSP/CMC dressing was as follows: 6 mg BSP, 14.4 mg CMC, 0.5 mg ST and 12 mg BA. The BA/ST/BSP/CMC dressing, containing antibacterial constituents, was non-cytotoxic and effective in accelerating the healing of burn wounds, making it a promising candidate for wound healing.

Please cite this article as: Gong YR, Zhang C, Xiang X, Wang ZB, Wang YQ, Su YH, Zhang HQ. Baicalin, silver titanate, Bletilla striata polysaccharide and carboxymethyl chitosan in a porous sponge dressing for burn wound healing. J Integr Med. 2023; 21(5): 487–495.

查看原文本刊更多论文

黄芩苷、钛酸银、白芷多糖和羧甲基壳聚糖在多孔海绵敷料中用于烧伤愈合。

目的：本研究测试了白芷多糖（BSP）、羧甲基壳聚糖（CMC）、黄芩苷（BA）和钛酸银（ST）在创伤敷料中对抗感染、促进愈合和提供良好生物相容性的功效。方法：采用抑菌带法测定BA和ST的体外抗菌活性。制备并表征了BA/ST/BSP/CMC多孔海绵敷料。使用细胞计数试剂盒-8测定BA/ST/BSP/CMC的生物相容性。采用Sprague-Dawley大鼠背部皮肤烧伤模型进一步研究了BA/ST/BSP/CMC的治疗作用。结果：伤口敷料通过BA和ST对大肠杆菌和金黄色葡萄球菌具有良好的抗菌活性，BSP和CMC联合使用对伤口愈合具有重要的促进作用。采用冷冻干燥法制备了BA/ST/BSP/CMC多孔海绵敷料，BA和ST的浓度分别为20和0.83mg/mL，5%BSP/4%CMC的最佳比例为1:3。BA/ST/BSP/CMC多孔海绵敷料的平均孔隙率、吸水率和透气性分别为90.43%、746.1%和66.60%。治疗3天和7天后，BA/ST/BSP/CMC组和BA/BSP/CCM组的愈合率均显著高于生理盐水组和磺胺嘧啶银组（P<0.05），白细胞介素1β在第1天和第3天的表达均显著低于其他三组（P）结论：BA／ST／BSP／CMC敷料的最佳浓度为：BSP 6 mg、CMC 14.4 mg、ST 0.5 mg、BA 12 mg。BA/ST/BSP/CMC敷料含有抗菌成分，无细胞毒性，能有效加速烧伤伤口的愈合，是一种很有前途的伤口愈合候选材料。请引用本文为：龚YR，张C，向X，王ZB，王YQ，苏YH，张HQ。黄芩苷、钛酸银、白芷多糖和羧甲基壳聚糖在多孔海绵敷料中用于烧伤愈合。国际医学杂志，2023；21（5）：487-495。

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来源期刊

Journal of Integrative Medicine-Jim Medicine-Complementary and Alternative Medicine

CiteScore

9.20

自引率

4.20%

发文量

3319

期刊介绍： The predecessor of JIM is the Journal of Chinese Integrative Medicine (Zhong Xi Yi Jie He Xue Bao). With this new, English-language publication, we are committed to make JIM an international platform for publishing high-quality papers on complementary and alternative medicine (CAM) and an open forum in which the different professions and international scholarly communities can exchange views, share research and their clinical experience, discuss CAM education, and confer about issues and problems in our various disciplines and in CAM as a whole in order to promote integrative medicine. JIM is indexed/abstracted in: MEDLINE/PubMed, ScienceDirect, Emerging Sources Citation Index (ESCI), Scopus, Embase, Chemical Abstracts (CA), CAB Abstracts, EBSCO, WPRIM, JST China, Chinese Science Citation Database (CSCD), and China National Knowledge Infrastructure (CNKI). JIM Editorial Office uses ThomsonReuters ScholarOne Manuscripts as submitting and review system (submission link: http://mc03.manuscriptcentral.com/jcim-en). JIM is published bimonthly. Manuscripts submitted to JIM should be written in English. Article types include but are not limited to randomized controlled and pragmatic trials, translational and patient-centered effectiveness outcome studies, case series and reports, clinical trial protocols, preclinical and basic science studies, systematic reviews and meta-analyses, papers on methodology and CAM history or education, conference proceedings, editorials, commentaries, short communications, book reviews, and letters to the editor. Our purpose is to publish a prestigious international journal for studies in integrative medicine. To achieve this aim, we seek to publish high-quality papers on any aspects of integrative medicine, such as acupuncture and traditional Chinese medicine, Ayurveda medicine, herbal medicine, homeopathy, nutrition, chiropractic, mind-body medicine, taichi, qigong, meditation, and any other modalities of CAM; our commitment to international scope ensures that research and progress from all regions of the world are widely covered. These ensure that articles published in JIM have the maximum exposure to the international scholarly community. JIM can help its authors let their papers reach the widest possible range of readers, and let all those who share an interest in their research field be concerned with their study.