Short-term and repeated exposure to particulate matter sizes from Imperial Valley, California to induce inflammation and asthmatic-like symptoms in mice.

IF 2.3 4区 医学 Q3 ENVIRONMENTAL SCIENCES
Savannah M D'Evelyn, Keith J Bein, Emilia A Laing, Tran Nyguen, Ching-Wen Wu, Qi Zhang, Kent E Pinkerton
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引用次数: 0

Abstract

Imperial Valley, California has become increasingly hot, dry, and polluted over the past decade. Particulate matter (PM) levels are amongst the highest in this State, associated with significantly higher asthma prevalence among children in the region compared to national and state averages. The present study was performed to test the hypothesis that Imperial Valley PM by size and chemical composition might possess allergenic properties following introduction into murine lungs without prior sensitization to a known allergen with size fraction as a determining factor. In acute exposure experiments, BALB/c male mice were administered a single 50-μl oropharyngeal aspiration of nanopure water (H2O; control) or a stock 1 μg/μl PM solution. In sub-acute exposure experiments, male and female mice were treated with a total of six 16.6-μl intranasal instillations of H2O or stock PM solution over the course of 14 days. In all experiments, pulmonary function tests were performed 24 hr after the final instillation followed by necropsies for the collection of biological samples. Inflammatory responses measured via cellularity in histopathological tissue sections as well as significant, marked influxes of eosinophils and lymphocytes were noted in the bronchoalveolar lavage fluid in mice administered PM compared to control. Allergic responses, including airway hyperresponsiveness and significantly increased expression of IL-1ß, were found in male mice exposed to either PM2.5 or ultrafine (PMUF). A combination of all three size fractions of PM from Imperial Valley initiated atopic and asthmatic-like symptoms in the lungs of mice in the absence of additional allergen or preexisting condition.

短期和反复暴露于来自加利福尼亚帝国谷的颗粒物大小,以诱导小鼠出现炎症和哮喘样症状。
在过去的十年里,加利福尼亚州的帝王谷变得越来越炎热、干燥和污染。颗粒物(PM)水平是该州最高的,与全国和州的平均水平相比,该地区儿童的哮喘患病率明显更高。本研究旨在检验以下假设,即帝国谷PM在引入小鼠肺部后,通过大小和化学成分可能具有致敏特性,而无需事先对已知过敏原致敏,大小分数是决定因素。在急性暴露实验中,给BALB/c雄性小鼠单次50μl口咽吸入纳米纯水(H2O;对照)或1 μg/μl PM溶液。在亚急性暴露实验中,雄性和雌性小鼠在14个疗程内总共用16.6μl的H2O或储备PM溶液鼻内滴注6次 天。在所有实验中,进行了肺功能测试24 最后滴注后小时,随后进行尸检以收集生物样品。与对照组相比,在给予PM的小鼠的支气管肺泡灌洗液中,通过组织病理学组织切片中的细胞密度测量的炎症反应以及嗜酸性粒细胞和淋巴细胞的显著显著流入。暴露于PM2.5或超细颗粒物(PMUF)的雄性小鼠出现过敏反应,包括气道高反应性和IL-1ß表达显著增加。在没有额外过敏原或预先存在的情况下,帝国谷PM的所有三种大小的组分的组合在小鼠的肺部引发特应性和哮喘样症状。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
5.20
自引率
19.20%
发文量
46
审稿时长
8-16 weeks
期刊介绍: The Journal of Toxicology and Environmental Health, Part A , Current Issues is an authoritative journal that features strictly refereed original research in the field of environmental sciences, public and occupational health, and toxicology.
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